Requirements for Human Respiratory Syncytial Virus Glycoproteins in Assembly and Egress from Infected Cells
Effect of the deletion of individual glycoprotein genes on colocalization of the HRSV nucleocapsid protein with each glycoprotein at the plasma membrane. (a) A diagram of an infected cell depicting HRSV assembly at the plasma membrane. The boxed area represents the 1 μm z-stack shown in subsequent confocal microscope images. The nucleus is depicted as a blue circle. A549 cells infected with WT or glycoprotein deleted HRSV at a MOI of 0.2 were fixed, permeabilized, and incubated with an anti-N antibody and an anti-F antibody (b), an anti-G antibody (c), or an anti-SH antibody (d). The glycoprotein stains are shown in the left row (red), the N protein stains in the middle row (green), and a merge of the two stains in the right row. A blowup of the merge is also shown. Images were taken on a Zeiss confocal microscope. An average of fourteen cells were imaged per staining, and colocalization was measured using ImageJ JACoP plugin. Pearson’s coefficient ± standard error of mean is shown within the merge panels.