Advances in Virology / 2011 / Article / Fig 4

Research Article

Requirements for Human Respiratory Syncytial Virus Glycoproteins in Assembly and Egress from Infected Cells

Figure 4

Effect of deletion of glycoprotein genes on the incorporation of HRSV proteins into released viral particles. Twenty-four hours after infection, A549 cells infected with WT or glycoprotein deleted HRSV at a MOI of 1.0 were radiolabeled with 35S Cys/Met for 16 h. The supernatant containing the released viral particles was collected, cleared of cell debris, and concentrated by centrifugation. (a) The viral pellet was resuspended, and viral proteins were immunoprecipitated with a polyclonal anti-HRSV antibody along with monoclonal antibodies against G and SH. Proteins were analyzed by electrophoresis on a 12% SDS polyacrylamide gel. A representative gel is shown. The bar graph depicts ratios of each HRSV structural protein to N protein, normalized to the ratios found in WT viral particles. (b) Infected cells were lysed and viral proteins were immunoprecipitated with a polyclonal anti-HRSV antibody along with antibodies against G and SH. Proteins were analyzed on a 12% SDS-PAGE. A representative gel is shown. The bar graph depicts ratios of each HRSV structural protein to N protein, normalized to the ratios found in WT-infected lysate. Proteins were quantitated on a phosphorImager. Error bars represent standard deviation from at least three experiments.
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