Review Article

XMRV Discovery and Prostate Cancer-Related Research

Figure 1

XMRV discovery in prostate cancer study [4]. Human prostate cancer tissues were collected in the operating room at the Cleveland Clinic and used to isolate RNA at either the Cleveland Clinic or at UCSF. At UCSF, RNA was used to synthesize labeled cDNA, virochips were probed, RT-PCR was performed for gag sequences, and XMRV cDNAs were sequenced. The RNase L genotypes were determined at the Cleveland Clinic (also the site of the IHC and FISH experiments, not shown). A hybridization pattern typical of a γretrovirus was obtained almost exclusively from patients with the RNase L QQ genotype (red bands and VP codes for QQ patients). RNase L RQ and RR genotypes are shown in black VP codes.
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