Internally Controlled, Generic Real-Time PCR for Quantification and Multiplex Real-Time PCR with Serotype-Specific Probes for Serotyping of Dengue Virus Infections

Table 2

Dynamic range of IC^a.

IC copies in extraction	IC copies in RT	IC copies in PCR^b	Number positives IC (%; mean Cq-value & SD)
1.25×10^7	10^7	10^6	4/4 (100%; 20.74; 0.23)
1.25×10^6	10^6	10^5	4/4 (100%; 24.80; 0.43)
1.25×10^5	10^5	10^4	4/4 (100%; 27.92; 0.48)
1.25×10^4	10^4	10^3	4/4 (100%; 32.08; 0.71)
1.25×10^3	10^3	10^2	4/4 (100%; 34.53; 0.56)
1.25×10^2	10^2	10^1	4/4 (100%; 36.93; 0.72)
0	0	0	0/4

^aThe dynamic range was determined on 10-fold serial dilution of IC spiked into lysis buffer containing DENV negative human serum as background with 4 replicates for each dilution.
^bNumber of copies cDNA/PCR has been calculated as 1/12.5 of the extracted RNA, assuming 100% efficiency in extraction, reverse transcription (RT), and PCR.
SD: standard deviation.