Internally Controlled, Generic Real-Time PCR for Quantification and Multiplex Real-Time PCR with Serotype-Specific Probes for Serotyping of Dengue Virus Infections

Table 7

Serotyping by multiplex real-time PCR with serotype-specific probes.

Serotype	No. of patients positive in the following assays			No. of infections by two serotypes detected by the following assays (DENV serotypes in infections)^c
	Type-specific PCR^a	Generic PCR	Multiplex PCR^b	Type-specific PCR^a	Multiplex PCR^b
1	39	Pos (39)	30	3 (1+2)	4 (1+4)
2	19	Pos (19)	17	0	1 (2+1)
3	3	Pos (3)	2	0	1 (3+4)
4	31	Pos (31)	31	0	1 (4+2)
total	92	92	80	3	7

Pos: generic PCR can make no distinction between the serotypes.
^aThe results were earlier generated by the type-specific primer PCR according to Laue et al. [11, 16, 17].
^bMultiplex real-time PCR with serotype-specific probes was only done on positive generic PCR results.
^cA DENV infection by two serotypes has been assigned to the serotype which signal came up first and showed the highest viral load of the two.