Research Article

Bacterial Toxin Fusion Proteins Elicit Mucosal Immunity against a Foot-and-Mouth Disease Virus Antigen When Administered Intranasally to Guinea Pigs

Figure 1

Generation and expression of fusion proteins. (a) Strategy for generation of recombinant fusion proteins by cloning amplicons from PCR reactions. (b) After construction a positive colony was selected (for each plasmid) and again screened for the presence of each fragments (ntPE, LTA2B, LTA2B-GH and the G-H loop) by PCR amplification. (c) Upon expression by IPTG and nickel column purification of proteins from pET-LTA2B-GH, and pET-ntPE-LTA2B-GH, western blots were performed using anti-G-H loop hyperimmune serum. Identified were proteins of approximately 15 and 78 kDa, respectively.
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