Figure 3: Kinetic comparison of HIV-1 B and CRF02_AG 33CR IN. (A) The kinetic features of recombinant HIV-1 B IN and (B) CRF02_AG IN (N1) were determined in vitro using 3′-processing activity assay, in the presence of 50, 100, 150, and 200 nM recombinant IN proteins with an incubation time of 10, 20, 30, 60, 90, 120, and 180 min, respectively.