Research Article

Elucidating the Interacting Domains of Chandipura Virus Nucleocapsid Protein

Figure 4

Screening of CHPV N1, N2, and N3 interactions with N, P, M, and G proteins by Y2H. All four viral genes cloned individually as DNA-binding domain fusion (bait) and transformed in Y187 yeast strain were systematically mated with N1, N2, and N3 cloned individually as DNA-activation domain fusions (prey), transformed in AH109 yeast strain. The diploid cells formed were selected on SD medium lacking amino acids tryptophan and leucine. Putative positive interactions were tested on SD media lacking tryptophan, leucine, and histidine All combinations were tested in at least 2 independent experiments. Presence of growth on medium (sectors 1, 2, 3, 4, 5, 8, 9, 10, and 11) indicated interaction between the proteins while absence (sectors 6, 7, and 12) suggested no interaction.
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