Mutations in the H, F, or M Proteins Can Facilitate Resistance of Measles Virus to Neutralizing Human Anti-MV Sera

Figure 3

Flow cytometry analysis of escape mutant Halle-CD46 1.3 (H: T177I, R533G; F: no change; M: I69T, I196V). Vero-SLAM cells infected with the escape mutant virus or transfected with phCMV plasmid expressing the mutated H, in the presence of antifusion tri-peptide FIP when necessary, were analysed 1 day after infection (or after transfection) using the indicated antibodies. Each overlay histogram represents one experiment; the MFI histogram data represent the mean percentages ± standard deviations for three experiments. These values were obtained by using nonmutated Halle virus or expressed nonmutated Halle-H protein as controls. (a) and (b)) Escape mutant Halle-CD46 1.3-infected cells; ((c) and (d)) and ((e) and (f)) cells transfected with phCMV.H.T177I/R533G and phCMV.H.R533G, respectively.