Table of Contents Author Guidelines Submit a Manuscript
Advances in Virology
Volume 2014, Article ID 274679, 3 pages
http://dx.doi.org/10.1155/2014/274679
Research Article

Detection of Influenza Virus Infection Using Two PCR Methods

1Department of Family Medicine, University of Pittsburgh School of Medicine, 3518 5th Avenue, Pittsburgh, PA 15261, USA
2Department of Pathology, University of Pittsburgh School of Medicine, PA 15261, USA
3Department of Infectious Disease and Microbiology, University of Pittsburgh Graduate School of Public Health, Pittsburgh, PA 15261, USA
4Department of Epidemiology, University of Pittsburgh Graduate School of Public Health, Pittsburgh, PA 15261, USA
5Influenza Division, Centers for Disease Control and Prevention, Atlanta, GA 30307, USA

Received 15 September 2014; Accepted 21 November 2014; Published 9 December 2014

Academic Editor: Stefan Pöhlmann

Copyright © 2014 Richard K. Zimmerman et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Rapid, accurate, and cost-effective methods to identify the cause of respiratory tract infections are needed to maximize clinical benefit. Outpatients with acute respiratory illness were tested for influenza using a singleplex reverse transcriptase polymerase chain reaction (SRT-PCR) method. A multiplex RT-PCR (MRT-PCR) method tested for influenza and 17 other viruses and was compared with SRT-PCR using chi-square tests. Among 935 patients, 335 (36%) tested positive for influenza A and influenza B using SRT-PCR. Using MRT-PCR, 320 (34.2%) tested positive for influenza A and influenza B. This study supports MRT-PCR as a comparable method for detecting influenza among patients seeking outpatient care for acute respiratory illnesses.