Research Article

Appearance of L90I and N205S Mutations in Effector Domain of NS1 Gene of pdm (09) H1N1 Virus from India during 2009–2013

Table 4

Selection pressure analysis of NS1 protein and NEP of H1N1 pdm (09) virus using SLAC, FEL, REL, MEME, and FUBAR methods at (http://www.datamonkey.org).

ProteinCodon*SLAC
N-S
SLAC
value
FEL
N-S
FEL
value
REL
N-S
REL Bayes
factor
MEME
ω+
MEME
value
FUBAR
N-S
FUBAR
post. pr.

NS15517.6140.13173.7940.0563.8983779.510>1000.0693.1510.989
1085.3300.45919.1330.2083.14911.971>1000.2510.2860.769
1235.3990.44724.4240.1423.55822.722>1000.2190.5420.813
12913.3330.14262.5220.0433.97819238.300>1000.0742.9930.992
1458.1030.63037.0710.3183.776514.262>1000.3281.2850.924
1477.4910.23234.0830.0843.82845.133>1000.1011.0810.880
2058.8700.43833.1930.1623.9282940.990>1000.1711.0420.922

NEP496.7290.457134.5020.1580.2602.013>1000.1831.8460.926

Significance value (SLAC value = 0.5, FEL value = 0.25, REL Bayes factor = 50, MEME value = 0.1, FUBAR posterior probability = 0.9).
*The sites found under positive selection by at least two methods are shown.
Sites present in NS1 host factor interaction domains are highlighted with bold font.