Evaluation of NxTAG Respiratory Pathogen Panel and Comparison with xTAG Respiratory Viral Panel Fast v2 and Film Array Respiratory Panel for Detecting Respiratory Pathogens in Nasopharyngeal Aspirates and Swine/Avian-Origin Influenza A Subtypes in Culture Isolates
Table 2
Performance of NxTAG RRP for detecting respiratory viruses in nasopharyngeal aspirates.
Viruses
NxTAG Pos/ Ref Pos
NxTAG Neg/ Ref Pos
NxTAG Pos/ Ref Neg
NxTAG Neg/ Ref Neg
Sensitivity % (95% Cl)
Specificity % (95% Cl)
PPV % (95% Cl)
NPV % (95% Cl)
Influenza A
16
0
1
116
100 (75.9–100)
99.1 (94.6–100)
94.1 (69.2–99.7)
100 (96.0–100)
H3
12
0
0
121
100 (69.9–100)
100 (96.2–100)
100 (69.9–100)
100 (96.2–100)
H1pdm09
3
0
1
129
100 (31.0–100)
99.2 (95.1–100)
75 (21.9–98.7)
100 (96.4–100)
H1
0
0
0
133
NA
NA
NA
NA
Influenza B
10
0
0
123
100 (65.5–100)
100 (96.2–100)
100 (65.5–100)
100 (95.5–100)
RSV A
9
0
1
123
100 (62.9–100)
99.2 (94.9–100)
90 (54.1–99.5)
100 (96.2–100)
RSV B
2
0
0
131
100 (19.8–100)
100 (96.4–100)
100 (19.8–100)
100 (96.4–100)
PIF1
10
0
1
122
100 (65.5–100)
99.2 (94.9–100)
99.9 (57.1–99.5)
100 (96.2–100)
PIF2
9
1
0
123
90.0 (54.1–99.4)
100 (96.2–100)
100 (62.9–100)
99.2 (94.9–100)
PIF3
10
0
0
123
100 (65.5–100)
100 (96.2–100)
100 (65.5–100)
100 (96.2–100)
PIF4
8
3
0
122
72.7 (39.0–92.6)
100 (96.2–100)
100 (59.8–100)
97.6 (92.6–99)
Adenovirus
4
0
1
128
100 (39.6–100)
99.2 (95.1–100)
80.0 (29.9–98.9)
100 (96.4–100)
hMPV
10
1
0
122
90.9 (57.1–99.5)
100 (96.2–100)
100 (66.5–100)
99.2 (94.9–100)
HCoV-229E
3
0
0
130
100 (31.0–100)
100 (96.4–100)
100 (31.0–100)
100 (96.4–100)
HCoV-OC43
6
0
0
127
100 (51.7–100)
100 (96.3–100)
100 (51.7–100)
100 (96.3–100)
HCoV-NL63
1
0
0
132
100 (5.5–100)
100 (96.5–100)
100 (5.5–100)
100 (96.5–100)
HCoV-HKU1
5
0
0
128
100 (46.3–100)
100 (96.4–100)
100 (46.3–100)
100 (96.4–100)
EV/rhinovirus
26
2
4
101
92.9 (75.0–98.8)
96.2 (90.0–98.8)
86.7 (68.4–95.6)
98.1 (92.4–100)
Bocavirus
9
0
2
122
100 (62.9–100)
98.4 (93.7–99.7)
81.8 (47.8–96.8)
100 (96.2–100)
C. pneumoniae
1
0
0
132
100 (5.5–100)
100 (96.5–100)
100 (5.5–100)
100 (96.5–100)
M. pneumoniae
3
1
0
129
75.0 (21.9–98.7)
100 (96.3–100)
100 (31.0–100)
99.2 (95.2–100)
L. pneumophila
1
0
0
132
100 (5.5–100)
100 (96.5–100)
100 (5.5–100)
100 (96.5–100)
B. pertussis
0
0
0
133
NA
NA
NA
NA
Total
158
8
11#
2882
95.2 (90.4–97.7)
99.6 (99.3–99.8)
93.5 (88.4–96.5)
99.7 (99.4–99.9)
EV, enterovirus; HCoV; human coronavirus; hMPV, human metapneumovirus; PIF, parainfluenza virus; PPV, positive predictive value; NPV, negative predictive value; RSV, respiratory syncytial virus, NA; not applicable; Ref, reference; Pos, positive; Neg, negative. #Overall total number of false positives is 10 because influenza A and subtyping were tested for a sample simultaneously.
