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Advances in Virology
Volume 2017, Article ID 6807964, 16 pages
https://doi.org/10.1155/2017/6807964
Review Article

Global Status of Porcine circovirus Type 2 and Its Associated Diseases in Sub-Saharan Africa

1SAMRC Microbial Water Quality Monitoring Centre, University of Fort Hare, Private Bag X1314, Alice 5700, Eastern Cape, South Africa
2Applied and Environmental Microbiology Research Group (AEMREG), Department of Biochemistry and Microbiology, University of Fort Hare, Private Bag X1314, Alice 5700, Eastern Cape, South Africa
3Academic and Research Division, University of Fort Hare, Private Bag X1314, Alice, Eastern Cape, South Africa

Correspondence should be addressed to Kayode O. Afolabi; moc.liamg@ibalofaakniyaloedoyak

Received 16 November 2016; Revised 13 February 2017; Accepted 23 February 2017; Published 12 March 2017

Academic Editor: Trudy Morrison

Copyright © 2017 Kayode O. Afolabi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. FAO, Pig Sector Kenya. FAO Animal Production and Health Livestock Country Reviews, No. 3, FAO, Rome, Italy, 2012.
  2. S. Uddin Khan, K. R. Atanasova, W. S. Krueger, A. Ramirez, and G. C. Gray, “Epidemiology, geographical distribution, and economic consequences of swine zoonoses: a narrative review,” Emerging Microbes and Infections, vol. 2, article e92, 2013. View at Publisher · View at Google Scholar · View at Scopus
  3. E. K. Ndyomugyenyi and J. Kyasimire, “Pig production in Kichwamba Sub-county, Rubirizi district, Uganda,” Livestock Research for Rural Development, vol. 27, no. 10, 2015. View at Google Scholar · View at Scopus
  4. M. M. Dione, E. A. Ouma, K. Roesel, J. Kungu, P. Lule, and D. Pezo, “Participatory assessment of animal health and husbandry practices in smallholder pig production systems in three high poverty districts in Uganda,” Preventive Veterinary Medicine, vol. 117, no. 3-4, pp. 565–576, 2014. View at Publisher · View at Google Scholar · View at Scopus
  5. P. M. P. Vidigal, C. L. Mafra, F. M. F. Silva, J. L. R. Fietto, A. Silva Júnior, and M. R. Almeida, “Tripping over emerging pathogens around the world: a phylogeographical approach for determining the epidemiology of Porcine circovirus-2 (PCV-2), considering global trading,” Virus Research, vol. 163, no. 1, pp. 320–327, 2012. View at Publisher · View at Google Scholar · View at Scopus
  6. P. Baekbo, C. S. Kristensen, and L. E. Larsen, “Porcine circovirus diseases: a review of PMWS,” Transboundary and Emerging Diseases, vol. 59, no. 1, pp. 60–67, 2012. View at Publisher · View at Google Scholar · View at Scopus
  7. X. Wang, K. He, W. Wang et al., “Genetic variation of porcine circovirus type 2 isolate 201105ZJ,” Agricultural Science and Technology, vol. 15, no. 11, pp. 1860–1864, 2014. View at Google Scholar
  8. C. Chae, “A review of porcine circovirus 2-associated syndromes and diseases,” Veterinary Journal, vol. 169, no. 3, pp. 326–336, 2005. View at Publisher · View at Google Scholar · View at Scopus
  9. G. M. Allan, S. Kennedy, F. McNeilly et al., “Experimental reproduction of severe wasting disease by co-infection of pigs with porcine circovirus and porcine parvovirus,” Journal of Comparative Pathology, vol. 121, no. 1, pp. 1–11, 1999. View at Publisher · View at Google Scholar · View at Scopus
  10. R. Anoopraj, T. K. Rajkhowa, S. Cherian et al., “Genetic characterisation and phylogenetic analysis of PCV2 isolates from India: indications for emergence of natural inter-genotypic recombinants,” Infection, Genetics and Evolution, vol. 31, pp. 25–32, 2015. View at Publisher · View at Google Scholar · View at Scopus
  11. I. Tischer, R. Rasch, and G. Tochtermann, “Characterization of papovavirus-and picornavirus-like particles in permanent pig kidney cell lines,” ZBL.BAKT.REIHE A, vol. 226, no. 2, pp. 153–167, 1974. View at Google Scholar · View at Scopus
  12. I. Tischer, H. Gelderblom, W. Vettermann, and M. A. Koch, “A very small porcine virus with circular single-stranded DNA,” Nature, vol. 295, no. 5844, pp. 64–66, 1982. View at Publisher · View at Google Scholar · View at Scopus
  13. G. M. Allan, F. McNeilly, J. P. Cassidy et al., “Pathogenesis of porcine circovirus; experimental infections of colostrum deprived piglets and examination of pig foetal material,” Veterinary Microbiology, vol. 44, no. 1, pp. 49–64, 1995. View at Publisher · View at Google Scholar · View at Scopus
  14. I. Tischer, W. Mields, D. Wolff, M. Vagt, and W. Griem, “Studies on epidemiology and pathogenicity of porcine circovirus,” Archives of Virology, vol. 91, no. 3-4, pp. 271–276, 1986. View at Publisher · View at Google Scholar · View at Scopus
  15. E. G. Clark, “Post-weaning multisystemic syndrome,” in Proceedings of the Western Canadian Association of Swine Practitioners Conference, pp. 22–25, 1996.
  16. J. Harding, “Post-weaning multisystemic wasting syndrome (PMWS): preliminary epidemiology and clinical presentation,” in Proceedings of the Western Canadian Association of Swine Practitioners, p. 21, 1996.
  17. J. Ellis, “Porcine circovirus: a historical perspective,” Veterinary Pathology, vol. 51, no. 2, pp. 315–327, 2014. View at Publisher · View at Google Scholar · View at Scopus
  18. A. Bratanich, M. Lairmore, W. Heneine et al., “Lack of evidence of conserved lentiviral sequences in pigs with post weaning multisystemic wasting syndrome,” Canadian Journal of Veterinary Research, vol. 63, no. 3, pp. 207–211, 1999. View at Google Scholar · View at Scopus
  19. G. M. Allan, F. McNeilly, S. Kennedy et al., “Isolation of porcine circovirus-like viruses from pigs with a wasting disease in the USA and Europe,” Journal of Veterinary Diagnostic Investigation, vol. 10, no. 1, pp. 3–10, 1998. View at Publisher · View at Google Scholar · View at Scopus
  20. J. C. S. Harding, E. G. Clark, J. H. Strokappe, P. I. Willson, and J. A. Ellis, “Postweaning multisystemic wasting syndrome: epidemiology and clinical presentation,” Journal of Swine Health and Production, vol. 6, no. 6, pp. 249–254, 1998. View at Google Scholar · View at Scopus
  21. J. Segalés, M. Sitjar, M. Domingo et al., “First report of post-weaning multisystemic wasting syndrome in pigs in Spain,” Veterinary Record, vol. 141, no. 23, pp. 600–601, 1997. View at Google Scholar · View at Scopus
  22. G. Allan, B. Meehan, D. Todd et al., “Novel porcine circoviruses from pigs with wasting disease syndromes,” The Veterinary Record, vol. 142, no. 17, pp. 467–468, 1998. View at Google Scholar · View at Scopus
  23. B. M. Meehan, F. McNeilly, D. Todd et al., “Characterization of novel circovirus DNAs associated with wasting syndromes in pigs,” Journal of General Virology, vol. 79, no. 9, pp. 2171–2179, 1998. View at Publisher · View at Google Scholar · View at Scopus
  24. G. M. Allan, F. Mc Neilly, B. M. Meehan et al., “Isolation and characterisation of circoviruses from pigs with wasting syndromes in Spain, Denmark and Northern Ireland,” Veterinary Microbiology, vol. 66, no. 2, pp. 115–123, 1999. View at Publisher · View at Google Scholar · View at Scopus
  25. J. Segalés, G. M. Allan, and M. Domingo, “Porcine circovirus diseases,” Animal health research reviews / Conference of Research Workers in Animal Diseases, vol. 6, no. 2, pp. 119–142, 2005. View at Publisher · View at Google Scholar · View at Scopus
  26. J. Segalés, “Porcine circovirus type 2 (PCV2) infections: clinical signs, pathology and laboratory diagnosis,” Virus Research, vol. 164, no. 1-2, pp. 10–19, 2012. View at Publisher · View at Google Scholar · View at Scopus
  27. N. Rose, T. Opriessnig, B. Grasland, and A. Jestin, “Epidemiology and transmission of porcine circovirus type 2 (PCV2),” Virus Research, vol. 164, no. 1-2, pp. 78–89, 2012. View at Publisher · View at Google Scholar · View at Scopus
  28. B. Jacobsen, L. Krueger, F. Seeliger, M. Bruegmann, J. Segalés, and W. Baumgaertner, “Retrospective study on the occurrence of porcine circovirus 2 infection and associated entities in Northern Germany,” Veterinary Microbiology, vol. 138, no. 1-2, pp. 27–33, 2009. View at Publisher · View at Google Scholar · View at Scopus
  29. L. Grau-Roma, L. Fraile, and J. Segalés, “Recent advances in the epidemiology, diagnosis and control of diseases caused by porcine circovirus type 2,” Veterinary Journal, vol. 187, no. 1, pp. 23–32, 2011. View at Publisher · View at Google Scholar · View at Scopus
  30. C. R. Pringle, “Virus taxonomy at the XIth International Congress of Virology, Sydney, Australia,” Archives of Virology, vol. 144, no. 10, pp. 2065–2070, 1999. View at Publisher · View at Google Scholar · View at Scopus
  31. M. McNulty, J. Dale, P. Lukert, A. Mankertz, J. Randles, and D. Todd, “Circoviridae,” in Seventh Report of the International Committee on Taxonomy of Viruses, M. H. V. van Regenmortel, C. M. Fauquet, D. H. L. Bishop et al., Eds., pp. 299–303, Academic Press, San Diego, Calif, USA, 2000. View at Google Scholar
  32. P. Biagini, M. Bendinelli, S. Hino et al., “Family circoviridae,” in Virus Taxonomy: Classification and Nomenclature of Viruses: Ninth Report of the International Committee on Taxonomy of Viruses, A. M. Q. King, M. J. Adams, E. B. Carstens, and E. J. Lefkowitz, Eds., pp. 343–349, Academic Press, London, UK, 2012. View at Google Scholar
  33. B. M. Meehan, J. L. Creelan, M. Stewart McNulty, and D. Todd, “Sequence of porcine circovirus DNA: affinities with plant circoviruses,” Journal of General Virology, vol. 78, pp. 221–227, 1997. View at Publisher · View at Google Scholar · View at Scopus
  34. F. D. Niagro, A. N. Forsthoefel, R. P. Lawther et al., “Beak and feather disease virus and porcine circovirus genomes: intermediates between the geminiviruses and plant circoviruses,” Archives of Virology, vol. 143, no. 9, pp. 1723–1744, 1998. View at Publisher · View at Google Scholar · View at Scopus
  35. R. Sanchez, H. Nauwynck, and M. Pensaert, “Serological survey of porcine circovirus 2 antibodies in domestic and feral pig populations in Belgium,” in Proceedings of the Conference on ssDNA Viruses of Plants, Birds, Pigs and Primates Meeting, p. 122, 2000.
  36. S. S. Grierson, D. P. King, T. Sandvik et al., “Detection and genetic typing of type 2 porcine circoviruses in archived pig tissues from the UK,” Archives of Virology, vol. 149, no. 6, pp. 1171–1183, 2004. View at Publisher · View at Google Scholar · View at Scopus
  37. S. Krakowka, G. Allan, J. Ellis et al., “A nine-base nucleotide sequence in the porcine circovirus type 2 (PCV2) nucleocapsid gene determines viral replication and virulence,” Virus Research, vol. 164, no. 1-2, pp. 90–99, 2012. View at Publisher · View at Google Scholar · View at Scopus
  38. I. W. Walker, C. A. Konoby, V. A. Jewhurst et al., “Development and application of a competitive enzyme linked immunosorbent assay for the detection of serum antibodies to porcine circovirus type 2,” Journal of Veterinary Diagnostic Investigation, vol. 12, no. 5, pp. 400–405, 2000. View at Publisher · View at Google Scholar · View at Scopus
  39. R. Magar, P. Müller, and R. Larochelle, “Retrospective serological survey of antibodies to porcine circovirus type 1 and type 2,” Canadian Journal of Veterinary Research, vol. 64, no. 3, pp. 184–186, 2000. View at Google Scholar · View at Scopus
  40. G. M. Rodríguez-Arrioja, J. Segalés, C. Rosell et al., “Retrospective study on porcine circovirus type 2 infection in pigs from 1985 to 1997 in Spain,” Journal of veterinary medicine. B, Infectious diseases and veterinary public health, vol. 50, no. 2, pp. 99–101, 2003. View at Publisher · View at Google Scholar · View at Scopus
  41. K. Rosario, M. Breitbart, B. Harrach et al., “Revisiting the taxonomy of the family Circoviridae: establishment of the genus Cyclovirus and removal of the genus Gyrovirus,” Archives of Virology, pp. 1–17, 2017. View at Publisher · View at Google Scholar
  42. E. Delwart and L. Li, “Rapidly expanding genetic diversity and host range of the Circoviridae viral family and other Rep encoding small circular ssDNA genomes,” Virus Research, vol. 164, no. 1-2, pp. 114–121, 2012. View at Publisher · View at Google Scholar · View at Scopus
  43. L. Li, A. Kapoor, B. Slikas et al., “Multiple diverse circoviruses infect farm animals and are commonly found in human and chimpanzee feces,” Journal of Virology, vol. 84, no. 4, pp. 1674–1682, 2010. View at Publisher · View at Google Scholar · View at Scopus
  44. I. Morozov, T. Sirinarumitr, S. D. Sorden et al., “Detection of a novel strain of porcine circovirus in pigs with postweaning multisystemic wasting syndrome,” Journal of Clinical Microbiology, vol. 36, no. 9, pp. 2535–2541, 1998. View at Google Scholar · View at Scopus
  45. A. Mankertz and B. Hillenbrand, “Analysis of transcription of porcine circovirus type 1,” Journal of General Virology, vol. 83, no. 11, pp. 2743–2751, 2002. View at Publisher · View at Google Scholar · View at Scopus
  46. S.-L. Zhai, S.-N. Chen, Z.-H. Xu et al., “Porcine circovirus type 2 in China: an update on and insights to its prevalence and control,” Virology Journal, vol. 11, article 88, 2014. View at Publisher · View at Google Scholar · View at Scopus
  47. A. Mankertz, J. Mankertz, K. Wolf, and H.-J. Buhk, “Identification of a protein essential for replication of porcine circovirus,” Journal of General Virology, vol. 79, no. 2, pp. 381–384, 1998. View at Publisher · View at Google Scholar · View at Scopus
  48. P. Nawagitgul, I. Morozov, S. R. Bolin, P. A. Harms, S. D. Sorden, and P. S. Paul, “Open reading frame 2 of porcine circovirus type 2 encodes a major capsid protein,” Journal of General Virology, vol. 81, no. 9, pp. 2281–2287, 2000. View at Publisher · View at Google Scholar · View at Scopus
  49. J. Liu, I. Chen, and J. Kwang, “Characterization of a previously unidentified viral protein in porcine circovirus type 2-infected cells and its role in virus-induced apoptosis,” Journal of Virology, vol. 79, no. 13, pp. 8262–8274, 2005. View at Publisher · View at Google Scholar · View at Scopus
  50. J. Liu, I. Chen, Q. Du, H. Chua, and J. Kwang, “The ORF3 protein of porcine circovirus type 2 is involved in viral pathogenesis in vivo,” Journal of Virology, vol. 80, no. 10, pp. 5065–5073, 2006. View at Publisher · View at Google Scholar · View at Scopus
  51. J. Gu, L. Wang, Y. Jin et al., “Characterization of specific antigenic epitopes and the nuclear export signal of the Porcine circovirus 2 ORF3 protein,” Veterinary Microbiology, vol. 184, pp. 40–50, 2016. View at Publisher · View at Google Scholar · View at Scopus
  52. J. He, J. Cao, N. Zhou, Y. Jin, J. Wu, and J. Zhou, “Identification and functional analysis of the novel ORF4 protein encoded by porcine circovirus type 2,” Journal of Virology, vol. 87, no. 3, pp. 1420–1429, 2013. View at Publisher · View at Google Scholar · View at Scopus
  53. L. Ojok, J. B. Okuni, C. Hohloch, W. Hecht, and M. Reinacher, “Detection and characterisation of porcine circovirus 2 from Ugandan pigs,” Indian Journal of Veterinary Pathology, vol. 37, no. 1, pp. 77–80, 2013. View at Google Scholar
  54. A. L. Hamel, L. L. Lin, C. Sachvie, E. Grudeski, and G. P. Nayar, “PCR detection and characterization of type-2 porcine circovirus,” Canadian Journal of Veterinary Research, vol. 64, no. 1, pp. 44–52, 2000. View at Google Scholar · View at Scopus
  55. G. Franzo, M. Cortey, A. Olvera et al., “Revisiting the taxonomical classification of Porcine Circovirus type 2 (PCV2): still a real challenge,” Virology Journal, vol. 12, article 131, 2015. View at Publisher · View at Google Scholar · View at Scopus
  56. L. J. Guo, Y. H. Lu, Y. W. Wei, L. P. Huang, and C. M. Liu, “Porcine circovirus type 2 (PCV2): genetic variation and newly emerging genotypes in China,” Virology Journal, vol. 7, article 273, 2010. View at Publisher · View at Google Scholar · View at Scopus
  57. J. Segalés, A. Olvera, L. Grau-Roma et al., “PCV-2 genotype definition and nomenclature,” Veterinary Record, vol. 162, no. 26, pp. 867–868, 2008. View at Publisher · View at Google Scholar · View at Scopus
  58. K. Dupont, E. O. Nielsen, P. Baekbo, and L. E. Larsen, “Genomic analysis of PCV2 isolates from Danish archives and a current PMWS case-control study supports a shift in genotypes with time,” Veterinary Microbiology, vol. 128, no. 1-2, pp. 56–64, 2008. View at Publisher · View at Google Scholar · View at Scopus
  59. G. Franzo, M. Cortey, A. M. M. G. D. Castro et al., “Genetic characterisation of Porcine circovirus type 2 (PCV2) strains from feral pigs in the Brazilian Pantanal: an opportunity to reconstruct the history of PCV2 evolution,” Veterinary Microbiology, vol. 178, no. 1-2, pp. 158–162, 2015. View at Publisher · View at Google Scholar · View at Scopus
  60. X. Liu, F.-X. Wang, H.-W. Zhu, N. Sun, and H. Wu, “Phylogenetic analysis of porcine circovirus type 2 (PCV2) isolates from China with high homology to PCV2c,” Archives of Virology, vol. 161, no. 6, pp. 1591–1599, 2016. View at Publisher · View at Google Scholar · View at Scopus
  61. C. Xiao, K. M. Harmon, P. G. Halbur, and T. Opriessnig, “PCV2d-2 is the predominant type of PCV2 DNA in pig samples collected in the U.S. during 2014–2016,” Veterinary Microbiology, vol. 197, pp. 72–77, 2016. View at Publisher · View at Google Scholar
  62. T. Kwon, D. Lee, S. J. Yoo, S. H. Je, J. Y. Shin, and Y. S. Lyoo, “Genotypic diversity of porcine circovirus type 2 (PCV2) and genotype shift to PCV2d in Korean pig population,” Virus Research, vol. 228, pp. 24–29, 2017. View at Publisher · View at Google Scholar
  63. C.-T. Xiao, P. G. Halbur, and T. Opriessnig, “Global molecular genetic analysis of porcine circovirus type 2 (PCV2) sequences confirms the presence of four main PCV2 genotypes and reveals a rapid increase of PCV2d,” Journal of General Virology, vol. 96, no. 7, pp. 1830–1841, 2015. View at Publisher · View at Google Scholar · View at Scopus
  64. C. Chae, “An emerging porcine circovirus type 2b mutant (mPCV2b) originally known as PCV2d,” Veterinary Journal, vol. 203, no. 1, pp. 6–9, 2015. View at Publisher · View at Google Scholar · View at Scopus
  65. M. Cortey, A. Olvera, L. Grau-Roma, and J. Segalés, “Further comments on porcine circovirus type 2 (PCV2) genotype definition and nomenclature,” Veterinary Microbiology, vol. 149, no. 3-4, pp. 522–523, 2011. View at Publisher · View at Google Scholar · View at Scopus
  66. T. Opriessnig, X. J. Meng, and P. G. Halbur, “Porcine circovirus type 2-associated disease: update on current terminology, clinical manifestations, pathogenesis, diagnosis, and intervention strategies,” Journal of Veterinary Diagnostic Investigation, vol. 19, no. 6, pp. 591–615, 2007. View at Publisher · View at Google Scholar · View at Scopus
  67. N. M. Beach and X.-J. Meng, “Efficacy and future prospects of commercially available and experimental vaccines against porcine circovirus type 2 (PCV2),” Virus Research, vol. 164, no. 1-2, pp. 33–42, 2012. View at Publisher · View at Google Scholar · View at Scopus
  68. G. M. Allan, F. McNeilly, M. McMenamy et al., “Temporal distribution of porcine circovirus 2 genogroups recovered from postweaning multisystemic wasting syndrome-affected and -nonaffected farms in Ireland and Northern Ireland,” Journal of Veterinary Diagnostic Investigation, vol. 19, no. 6, pp. 668–673, 2007. View at Publisher · View at Google Scholar · View at Scopus
  69. M. Constans, M. Ssemadaali, O. Kolyvushko, and S. Ramamoorthy, “Antigenic determinants of possible vaccine escape by porcine circovirus subtype 2b viruses,” Bioinformatics and Biology Insights, vol. 9, pp. 1–12, 2015. View at Google Scholar · View at Scopus
  70. M. Cortey, E. Pileri, M. Sibila et al., “Genotypic shift of porcine circovirus type 2 from PCV-2a to PCV-2b in Spain from 1985 to 2008,” Veterinary Journal, vol. 187, no. 3, pp. 363–368, 2011. View at Publisher · View at Google Scholar · View at Scopus
  71. C. A. Gagnon, D. Tremblay, P. Tijssen, M.-H. Venne, A. Houde, and S. M. Elahi, “The emergence of porcine circovirus 2b genotype (PCV-2b) in swine in Canada,” Canadian Veterinary Journal, vol. 48, no. 8, pp. 811–819, 2007. View at Google Scholar · View at Scopus
  72. L. Huang, Y. Wang, Y. Wei et al., “Capsid proteins from PCV2a genotype confer greater protection against a PCV2b strain than those from PCV2b genotype in pigs: evidence for PCV2b strains becoming more predominant than PCV2a strains from 2000 to 2010s,” Applied Microbiology and Biotechnology, vol. 100, no. 13, pp. 5933–5943, 2016. View at Publisher · View at Google Scholar · View at Scopus
  73. T. Opriessnig, S. Ramamoorthy, D. M. Madson et al., “Differences in virulence among porcine circovirus type 2 isolates are unrelated to cluster type 2a or 2b and prior infection provides heterologous protection,” Journal of General Virology, vol. 89, no. 10, pp. 2482–2491, 2008. View at Publisher · View at Google Scholar · View at Scopus
  74. K. M. Lager, P. C. Gauger, A. L. Vincent, T. Opriessnig, M. E. Kehrli Jr., and A. K. Cheung, “Mortality in pigs given porcine circovirus type 2 subgroup 1 and 2 viruses derived from DNA clones,” Veterinary Record, vol. 161, no. 12, pp. 428–429, 2007. View at Publisher · View at Google Scholar · View at Scopus
  75. A. Tomás, L. T. Fernandes, O. Valero, and J. Segalés, “A meta-analysis on experimental infections with porcine circovirus type 2 (PCV2),” Veterinary Microbiology, vol. 132, no. 3-4, pp. 260–273, 2008. View at Publisher · View at Google Scholar · View at Scopus
  76. D. J. Lefebvre, S. Costers, J. Van Doorsselaere, G. Misinzo, P. L. Delputte, and H. J. Nauwynck, “Antigenic differences among porcine circovirus type 2 strains, as demonstrated by the use of monoclonal antibodies,” Journal of General Virology, vol. 89, no. 1, pp. 177–187, 2008. View at Publisher · View at Google Scholar · View at Scopus
  77. S.-B. Shang, Y.-L. Jin, X.-T. Jiang et al., “Fine mapping of antigenic epitopes on capsid proteins of porcine circovirus, and antigenic phenotype of porcine circovirus Type 2,” Molecular Immunology, vol. 46, no. 3, pp. 327–334, 2009. View at Publisher · View at Google Scholar · View at Scopus
  78. A. K. Cheung, K. M. Lager, O. I. Kohutyuk et al., “Detection of two porcine circovirus type 2 genotypic groups in United States swine herds,” Archives of Virology, vol. 152, no. 5, pp. 1035–1044, 2007. View at Publisher · View at Google Scholar · View at Scopus
  79. A. Olvera, M. Cortey, and J. Segalés, “Molecular evolution of porcine circovirus type 2 genomes: phylogeny and clonality,” Virology, vol. 357, no. 2, pp. 175–185, 2007. View at Publisher · View at Google Scholar · View at Scopus
  80. L. Guo, Y. Fu, Y. Wang et al., “A porcine circovirus type 2 (PCV2) mutant with 234 amino acids in Capsid protein showed more virulence in vivo, compared with classical PCV2a/b strain,” PLoS ONE, vol. 7, no. 7, Article ID e41463, 2012. View at Publisher · View at Google Scholar · View at Scopus
  81. T. Opriessnig, C.-T. Xiao, P. F. Gerber, and P. G. Halbur, “Emergence of a novel mutant PCV2b variant associated with clinical PCVAD in two vaccinated pig farms in the U.S. concurrently infected with PPV2,” Veterinary Microbiology, vol. 163, no. 1-2, pp. 177–183, 2013. View at Publisher · View at Google Scholar · View at Scopus
  82. C.-T. Xiao, P. G. Halbur, and T. Opriessnig, “Complete genome sequence of a novel porcine circovirus type 2b variant present in cases of vaccine failures in the United States,” Journal of Virology, vol. 86, no. 22, pp. 12469–12469, 2012. View at Publisher · View at Google Scholar · View at Scopus
  83. H. W. Seo, J. Lee, K. Han, C. Park, and C. Chae, “Comparative analyses of humoral and cell-mediated immune responses upon vaccination with different commercially available single-dose porcine circovirus type 2 vaccines,” Research in Veterinary Science, vol. 97, no. 1, pp. 38–42, 2014. View at Publisher · View at Google Scholar · View at Scopus
  84. R. L. Salgado, P. M. Vidigal, L. F. de Souza et al., “Identification of an emergent porcine circovirus-2 in vaccinated pigs from a brazilian farm during a postweaning multisystemic wasting syndrome outbreak,” Genome Announcements, vol. 2, no. 2, 2014. View at Publisher · View at Google Scholar
  85. N. Ramos, S. Mirazo, G. Castro, and J. Arbiza, “First identification of Porcine Circovirus Type 2b mutant in pigs from Uruguay,” Infection, Genetics and Evolution, vol. 33, pp. 320–323, 2015. View at Publisher · View at Google Scholar · View at Scopus
  86. M. Eddicks, R. Fux, F. Szikora et al., “Detection of a new cluster of porcine circovirus type 2b strains in domestic pigs in Germany,” Veterinary Microbiology, vol. 176, no. 3-4, pp. 337–343, 2015. View at Publisher · View at Google Scholar · View at Scopus
  87. G. Reiner, R. Hofmeister, and H. Willems, “Genetic variability of porcine circovirus 2 (PCV2) field isolates from vaccinated and non-vaccinated pig herds in Germany,” Veterinary Microbiology, vol. 180, no. 1-2, pp. 41–48, 2015. View at Publisher · View at Google Scholar · View at Scopus
  88. N. E. McKeown, T. Opriessnig, P. Thomas et al., “Effects of porcine circovirus type 2 (PCV2) maternal antibodies on experimental infection of piglets with PCV2,” Clinical and Diagnostic Laboratory Immunology, vol. 12, no. 11, pp. 1347–1351, 2005. View at Publisher · View at Google Scholar · View at Scopus
  89. J. Gillespie, T. Opriessnig, X. J. Meng, K. Pelzer, and V. Buechner-Maxwell, “Porcine circovirus type 2 and porcine circovirus-associated disease,” Journal of Veterinary Internal Medicine, vol. 23, no. 6, pp. 1151–1163, 2009. View at Publisher · View at Google Scholar · View at Scopus
  90. X.-J. Meng, “Porcine circovirus type 2 (PCV2): pathogenesis and interaction with the immune system,” Annual Review of Animal Biosciences, vol. 1, pp. 43–64, 2013. View at Publisher · View at Google Scholar · View at Scopus
  91. C. Chae, “Porcine circovirus type 2 and its associated diseases in Korea,” Virus Research, vol. 164, no. 1-2, pp. 107–113, 2012. View at Publisher · View at Google Scholar · View at Scopus
  92. J.-S. Park, J. Kim, Y. Ha et al., “Birth abnormalities in pregnant sows infected intranasally with porcine circovirus 2,” Journal of Comparative Pathology, vol. 132, no. 2-3, pp. 139–144, 2005. View at Publisher · View at Google Scholar · View at Scopus
  93. C. Salogni, M. Lazzaro, E. Giacomini et al., “Infectious agents identified in aborted swine fetuses in a high-density breeding area: A Three-Year Study,” Journal of Veterinary Diagnostic Investigation, vol. 28, no. 5, pp. 550–554, 2016. View at Publisher · View at Google Scholar
  94. P. A. Harms, S. D. Sorden, P. G. Halbur et al., “Experimental reproduction of severe disease in CD/CD pigs concurrently infected with type 2 porcine circovirus and porcine reproductive and respiratory syndrome virus,” Veterinary Pathology, vol. 38, no. 5, pp. 528–539, 2001. View at Publisher · View at Google Scholar · View at Scopus
  95. J. Kim, H.-K. Chung, and C. Chae, “Association of porcine circovirus 2 with porcine respiratory disease complex,” Veterinary Journal, vol. 166, no. 3, pp. 251–256, 2003. View at Publisher · View at Google Scholar · View at Scopus
  96. C. Rosell, J. Segalés, J. A. Ramos-Vara et al., “Identification of porcine circovirus in tissues of pigs with porcine dermatitis and nephropathy syndrome,” Veterinary Record, vol. 146, no. 2, pp. 40–43, 2000. View at Publisher · View at Google Scholar · View at Scopus
  97. G. J. Wellenberg, N. Stockhofe-Zurwieden, M. F. De Jong, W. J. A. Boersma, and A. R. W. Elbers, “Excessive porcine circovirus type 2 antibody titres may trigger the development of porcine dermatitis and nephropathy syndrome: A Case-Control Study,” Veterinary Microbiology, vol. 99, no. 3-4, pp. 203–214, 2004. View at Publisher · View at Google Scholar · View at Scopus
  98. J. Kim, Y. Ha, K. Jung, C. Choi, and C. Chae, “Enteritis associated with porcine circovirus 2 in pigs,” Canadian Journal of Veterinary Research, vol. 68, no. 3, pp. 218–221, 2004. View at Google Scholar · View at Scopus
  99. G. W. Stevenson, M. Kiupel, S. K. Mittal, J. Choi, K. S. Latimer, and C. L. Kanitz, “Tissue distribution and genetic typing of porcine circoviruses in pigs with naturally occurring congenital tremors,” Journal of Veterinary Diagnostic Investigation, vol. 13, no. 1, pp. 57–62, 2001. View at Publisher · View at Google Scholar · View at Scopus
  100. Y. Ha, K. Jung, and C. Chae, “Lack of evidence of porcine circovirus type 1 and type 2 infection in piglets with congenital tremors in Korea,” Veterinary Record, vol. 156, no. 12, pp. 383–384, 2005. View at Publisher · View at Google Scholar · View at Scopus
  101. S. Kennedy, J. Segalés, A. Rovira et al., “Absence of evidence of porcine circovirus infection in piglets with congenital tremors,” Journal of Veterinary Diagnostic Investigation, vol. 15, no. 2, pp. 151–156, 2003. View at Publisher · View at Google Scholar · View at Scopus
  102. P. Tummaruk and P. Pearodwong, “Porcine circovirus type 2 expression in the brain of neonatal piglets with congenital tremor,” Comparative Clinical Pathology, vol. 25, no. 4, pp. 727–732, 2016. View at Publisher · View at Google Scholar · View at Scopus
  103. F. Hasslung, P. Wallgren, A.-S. Ladekjær-Hansen et al., “Experimental reproduction of postweaning multisystemic wasting syndrome (PMWS) in pigs in Sweden and Denmark with a Swedish isolate of porcine circovirus type 2,” Veterinary Microbiology, vol. 106, no. 1-2, pp. 49–60, 2005. View at Publisher · View at Google Scholar · View at Scopus
  104. T. Opriessnig and P. G. Halbur, “Concurrent infections are important for expression of porcine circovirus associated disease,” Virus Research, vol. 164, no. 1-2, pp. 20–32, 2012. View at Publisher · View at Google Scholar · View at Scopus
  105. Y. Li, H. Liu, P. Wang et al., “RNA-seq analysis reveals genes underlying different disease responses to porcine circovirus type 2 in pigs,” PLoS ONE, vol. 11, no. 5, Article ID e0155502, 2016. View at Publisher · View at Google Scholar · View at Scopus
  106. R. Patterson, A. Nevel, A. V. Diaz et al., “Exposure to environmental stressors result in increased viral load and further reduction of production parameters in pigs experimentally infected with PCV2b,” Veterinary Microbiology, vol. 177, no. 3-4, pp. 261–269, 2015. View at Publisher · View at Google Scholar · View at Scopus
  107. S. Kennedy, D. Moffett, F. McNeilly et al., “Reproduction of lesions of postweaning multisystemic wasting syndrome by infection of conventional pigs with porcine circovirus type 2 alone or in combination with porcine parvovirus,” Journal of Comparative Pathology, vol. 122, no. 1, pp. 9–24, 2000. View at Publisher · View at Google Scholar · View at Scopus
  108. B. Lukač, A. Knežević, N. Milić et al., “Molecular detection of PCV2 and PPV in pigs in Republic of Srpska, Bosnia and Herzegovina,” Acta Veterinaria, vol. 66, no. 1, pp. 51–60, 2016. View at Publisher · View at Google Scholar · View at Scopus
  109. J. Sun, L. Huang, Y. Wei et al., “Prevalence of emerging porcine parvoviruses and their co-infections with porcine circovirus type 2 in China,” Archives of Virology, vol. 160, no. 5, pp. 1339–1344, 2015. View at Publisher · View at Google Scholar · View at Scopus
  110. G. M. Allan, F. McNeilly, J. Ellis et al., “Experimental infection of colostrum deprived piglets with porcine circovirus 2 (PCV2) and porcine reproductive and respiratory syndrome virus (PRRSV) potentiates PCV2 replication,” Archives of Virology, vol. 145, no. 11, pp. 2421–2429, 2000. View at Publisher · View at Google Scholar · View at Scopus
  111. P. M. Dorr, R. B. Baker, G. W. Almond, S. R. Wayne, and W. A. Gebreyes, “Epidemiologic assessment of porcine circovirus type 2 coinfection with other pathogens in swine,” Journal of the American Veterinary Medical Association, vol. 230, no. 2, pp. 244–250, 2007. View at Publisher · View at Google Scholar · View at Scopus
  112. Y. Yang, R. Shi, R. She et al., “Fatal disease associated with Swine Hepatitis E virus and Porcine circovirus 2 co-infection in four weaned pigs in China,” BMC Veterinary Research, vol. 11, no. 1, article 77, 2015. View at Publisher · View at Google Scholar · View at Scopus
  113. T. Opriessnig, E. L. Thacker, S. Yu, M. Fenaux, X.-J. Meng, and P. G. Halbur, “Experimental reproduction of postweaning multisystemic wasting syndrome in pigs by dual infection with Mycoplasma hyopneumoniae and porcine circovirus type 2,” Veterinary Pathology, vol. 41, no. 6, pp. 624–640, 2004. View at Publisher · View at Google Scholar · View at Scopus
  114. Y. Ha, K. Jung, K. Kim, C. Choi, and C. Chae, “Outbreak of salmonellosis in pigs with postweaning multisystemic wasting syndrome,” Veterinary Record, vol. 156, no. 18, pp. 583–584, 2005. View at Publisher · View at Google Scholar · View at Scopus
  115. C. E. Dewey, W. T. Johnston, L. Gould, and T. L. Whiting, “Postweaning mortality in Manitoba swine,” Canadian Journal of Veterinary Research, vol. 70, no. 3, pp. 161–167, 2006. View at Google Scholar · View at Scopus
  116. G. Marruchella, B. Paoletti, R. Speranza, and G. Di Guardo, “Fatal bronchopneumonia in a Metastrongylus elongatus and Porcine circovirus type 2 co-infected pig,” Research in Veterinary Science, vol. 93, no. 1, pp. 310–312, 2012. View at Publisher · View at Google Scholar · View at Scopus
  117. X.-J. Meng, “Spread like a wildfire—the omnipresence of porcine circovirus type 2 (PCV2) and its ever-expanding association with diseases in pigs,” Virus Research, vol. 164, no. 1-2, pp. 1–3, 2012. View at Publisher · View at Google Scholar · View at Scopus
  118. G. Franzo, C. M. Tucciarone, G. Dotto, A. Gigli, L. Ceglie, and M. Drigo, “International trades, local spread and viral evolution: the case of porcine circovirus type 2 (PCV2) strains heterogeneity in Italy,” Infection, Genetics and Evolution, vol. 32, pp. 409–415, 2015. View at Publisher · View at Google Scholar · View at Scopus
  119. T. W. Drew, S. S. Grierson, D. P. King et al., “Genetic similarity between porcine circovirus type 2 isolated from the first reported case of PMWS in South Africa and North American isolates,” Veterinary Record, vol. 155, no. 5, pp. 149–151, 2004. View at Publisher · View at Google Scholar · View at Scopus
  120. V. R. Monger, J. A. Stegeman, G. Koop, K. Dukpa, T. Tenzin, and W. L. A. Loeffen, “Seroprevalence and associated risk factors of important pig viral diseases in Bhutan,” Preventive Veterinary Medicine, vol. 117, no. 1, pp. 222–232, 2014. View at Publisher · View at Google Scholar · View at Scopus
  121. N. Rose, G. Larour, G. Le Diguerher et al., “Risk factors for porcine post-weaning multisystemic wasting syndrome (PMWS) in 149 French farrow-to-finish herds,” Preventive Veterinary Medicine, vol. 61, no. 3, pp. 209–225, 2003. View at Publisher · View at Google Scholar · View at Scopus
  122. K. Dupont, C. K. Hjulsager, C. S. Kristensen, P. Baekbo, and L. E. Larsen, “Transmission of different variants of PCV2 and viral dynamics in a research facility with pigs mingled from PMWS-affected herds and non-affected herds,” Veterinary Microbiology, vol. 139, no. 3-4, pp. 219–226, 2009. View at Publisher · View at Google Scholar · View at Scopus
  123. H. Shen, C. Wang, D. M. Madson, and T. Opriessnig, “High prevalence of porcine circovirus viremia in newborn piglets in five clinically normal swine breeding herds in North America,” Preventive Veterinary Medicine, vol. 97, no. 3-4, pp. 228–236, 2010. View at Publisher · View at Google Scholar · View at Scopus
  124. P. Alarcon, M. Velasova, A. Mastin, A. Nevel, K. D. C. Stärk, and B. Wieland, “Farm level risk factors associated with severity of post-weaning multi-systemic wasting syndrome,” Preventive Veterinary Medicine, vol. 101, no. 3-4, pp. 182–191, 2011. View at Publisher · View at Google Scholar · View at Scopus
  125. M. Lőrincz, Á. Cságola, I. Biksi, L. Szeredi, Á. Dán, and T. Tuboly, “Detection of porcine circovirus in rodents—short communication,” Acta Veterinaria Hungarica, vol. 58, no. 2, pp. 265–268, 2010. View at Publisher · View at Google Scholar · View at Scopus
  126. R. Blunt, S. McOrist, J. McKillen, I. McNair, T. Jiang, and K. Mellits, “House fly vector for porcine circovirus 2b on commercial pig farms,” Veterinary Microbiology, vol. 149, no. 3-4, pp. 452–455, 2011. View at Publisher · View at Google Scholar · View at Scopus
  127. X. Yang, L. Hou, J. Ye, Q. He, and S. Cao, “Detection of porcine circovirus type 2 (PCV2) in mosquitoes from pig farms by PCR,” Pakistan Veterinary Journal, vol. 32, no. 1, pp. 134–135, 2012. View at Google Scholar · View at Scopus
  128. D. Verreault, V. Létourneau, L. Gendron, D. Massé, C. A. Gagnon, and C. Duchaine, “Airborne porcine circovirus in Canadian swine confinement buildings,” Veterinary Microbiology, vol. 141, no. 3-4, pp. 224–230, 2010. View at Publisher · View at Google Scholar · View at Scopus
  129. M.-T. Chiou, C.-Y. Yang, T.-C. Chang, C. Chen, C.-F. Lin, and L.-J. Ye, “Shedding pattern and serological profile of porcine circovirus type 2 infection in cesarean-derived, colostrum-deprived and farm-raised pigs,” Journal of Veterinary Medical Science, vol. 73, no. 4, pp. 521–525, 2011. View at Publisher · View at Google Scholar · View at Scopus
  130. A. R. Patterson, D. M. Madson, P. G. Halbur, and T. Opriessnig, “Shedding and infection dynamics of porcine circovirus type 2 (PCV2) after natural exposure,” Veterinary Microbiology, vol. 149, no. 1-2, pp. 225–229, 2011. View at Publisher · View at Google Scholar · View at Scopus
  131. A. R. Patterson, S. Ramamoorthy, D. M. Madson, X. J. Meng, P. G. Halbur, and T. Opriessnig, “Shedding and infection dynamics of porcine circovirus type 2 (PCV2) after experimental infection,” Veterinary Microbiology, vol. 149, no. 1-2, pp. 91–98, 2011. View at Publisher · View at Google Scholar · View at Scopus
  132. J. Segalés, M. Calsamiglia, A. Olvera, M. Sibila, L. Badiella, and M. Domingo, “Quantification of porcine circovirus type 2 (PCV2) DNA in serum and tonsillar, nasal, tracheo-bronchial, urinary and faecal swabs of pigs with and without postweaning multisystemic wasting syndrome (PMWS),” Veterinary Microbiology, vol. 111, no. 3-4, pp. 223–229, 2005. View at Publisher · View at Google Scholar · View at Scopus
  133. I. Shibata, Y. Okuda, S. Yazawa et al., “PCR detection of Porcine circovirus type 2 DNA in whole blood, serum, oropharyngeal swab, nasal swab, and feces from experimentally infected pigs and field cases,” Journal of Veterinary Medical Science, vol. 65, no. 3, pp. 405–408, 2003. View at Publisher · View at Google Scholar · View at Scopus
  134. C. Liu, T. Ihara, T. Nunoya, and S. Ueda, “Development of an ELISA based on the baculovirus-expressed capsid protein of porcine circovirus type 2 as antigen,” Journal of Veterinary Medical Science, vol. 66, no. 3, pp. 237–242, 2004. View at Publisher · View at Google Scholar · View at Scopus
  135. M. Sibila, M. Calsamiglia, J. Segales et al., “Use of a polymerase chain reaction assay and an ELISA to monitor porcine circovirus type 2 infection in pigs from farms with and without postweaning multisystemic wasting syndrome,” American Journal of Veterinary Research, vol. 65, no. 1, pp. 88–92, 2004. View at Publisher · View at Google Scholar · View at Scopus
  136. H. Feng, G. Blanco, J. Segalés, and M. Sibila, “Can Porcine circovirus type 2 (PCV2) infection be eradicated by mass vaccination?” Veterinary Microbiology, vol. 172, no. 1-2, pp. 92–99, 2014. View at Publisher · View at Google Scholar · View at Scopus
  137. L. Fraile, M. Sibila, M. Nofrarías et al., “Effect of sow and piglet porcine circovirus type 2 (PCV2) vaccination on piglet mortality, viraemia, antibody titre and production parameters,” Veterinary Microbiology, vol. 161, no. 1-2, pp. 229–234, 2012. View at Publisher · View at Google Scholar · View at Scopus
  138. P. F. Gerber, F. M. Garrocho, Â. M. Q. Lana, and Z. I. P. Lobato, “Serum antibodies and shedding of infectious porcine circovirus 2 into colostrum and milk of vaccinated and unvaccinated naturally infected sows,” Veterinary Journal, vol. 188, no. 2, pp. 240–242, 2011. View at Publisher · View at Google Scholar · View at Scopus
  139. J. Segalés, A. Urniza, A. Alegre et al., “A genetically engineered chimeric vaccine against porcine circovirus type 2 (PCV2) improves clinical, pathological and virological outcomes in postweaning multisystemic wasting syndrome affected farms,” Vaccine, vol. 27, no. 52, pp. 7313–7321, 2009. View at Publisher · View at Google Scholar · View at Scopus
  140. T. Kekarainen, K. McCullough, M. Fort, C. Fossum, J. Segalés, and G. M. Allan, “Immune responses and vaccine-induced immunity against Porcine circovirus type 2,” Veterinary Immunology and Immunopathology, vol. 136, no. 3-4, pp. 185–193, 2010. View at Publisher · View at Google Scholar · View at Scopus
  141. S. Carman, H. Y. Cai, J. DeLay et al., “The emergence of a new strain of porcine circovirus-2 in Ontario and Quebec swine and its association with severe porcine circovirus associated disease—2004–2006,” Canadian Journal of Veterinary Research, vol. 72, no. 3, pp. 259–268, 2008. View at Google Scholar · View at Scopus
  142. T. Opriessnig, K. O'Neill, P. F. Gerber et al., “A PCV2 vaccine based on genotype 2b is more effective than a 2a-based vaccine to protect against PCV2b or combined PCV2a/2b viremia in pigs with concurrent PCV2, PRRSV and PPV infection,” Vaccine, vol. 31, no. 3, pp. 487–494, 2013. View at Publisher · View at Google Scholar · View at Scopus
  143. T. Opriessnig, C. T. Xiao, P. F. Gerber, and P. G. Halbur, “Identification of recently described porcine parvoviruses in archived North American samples from 1996 and association with porcine circovirus associated disease,” Veterinary Microbiology, vol. 173, no. 1-2, pp. 9–16, 2014. View at Publisher · View at Google Scholar · View at Scopus
  144. C. Firth, M. A. Charleston, S. Duffy, B. Shapiro, and E. C. Holmes, “Insights into the evolutionary history of an emerging livestock pathogen: porcine circovirus 2,” Journal of Virology, vol. 83, no. 24, pp. 12813–12821, 2009. View at Publisher · View at Google Scholar · View at Scopus
  145. M. A. Ssemadaali, M. Ilha, and S. Ramamoorthy, “Genetic diversity of porcine circovirus type 2 and implications for detection and control,” Research in Veterinary Science, vol. 103, pp. 179–186, 2015. View at Publisher · View at Google Scholar · View at Scopus
  146. Z. Peng, T. Ma, D. Pang et al., “Expression, purification and antibody preparation of PCV2 Rep and ORF3 proteins,” International Journal of Biological Macromolecules, vol. 86, pp. 277–281, 2016. View at Publisher · View at Google Scholar · View at Scopus
  147. Z. Afghah, B. Webb, X. Meng, and S. Ramamoorthy, “Ten years of PCV2 vaccines and vaccination: is eradication a possibility?” Veterinary Microbiology, 2016. View at Publisher · View at Google Scholar
  148. N. Sun, T. Yu, J.-X. Zhao et al., “Antiviral activities of natural compounds derived from traditional chinese medicines against porcine circovirus type 2 (PCV2),” Biotechnology and Bioprocess Engineering, vol. 20, no. 1, pp. 180–187, 2015. View at Publisher · View at Google Scholar · View at Scopus
  149. J. Segalés, T. Kekarainen, and M. Cortey, “The natural history of porcine circovirus type 2: from an inoffensive virus to a devastating swine disease?” Veterinary Microbiology, vol. 165, no. 1-2, pp. 13–20, 2013. View at Publisher · View at Google Scholar · View at Scopus
  150. N. Sun, P. Sun, H. Lv et al., “Matrine displayed antiviral activity in porcine alveolar macrophages co-infected by porcine reproductive and respiratory syndrome virus and porcine circovirus type 2,” Scientific Reports, vol. 6, Article ID 24401, 2016. View at Publisher · View at Google Scholar · View at Scopus
  151. J. Chen, W. Li, E. Jin et al., “The antiviral activity of arctigenin in traditional Chinese medicine on porcine circovirus type 2,” Research in Veterinary Science, vol. 106, pp. 159–164, 2016. View at Publisher · View at Google Scholar · View at Scopus
  152. J. Ellis, L. Hassard, E. Clark et al., “Isolation of circovirus from lesions of pigs with postweaning multisystemic wasting syndrome,” Canadian Veterinary Journal, vol. 39, no. 1, pp. 44–51, 1998. View at Google Scholar · View at Scopus
  153. J. C. Harding and E. Clark, “Recognizing and diagnosing postweaning multisystemic wasting syndrome (PMWS),” Journal of Swine Health Production, vol. 5, pp. 201–203, 1997. View at Google Scholar
  154. P. LeCann, E. Albina, F. Madec, R. Cariolet, and A. Jestin, “Piglet wasting disease,” The Veterinary Record, vol. 141, no. 25, p. 660, 1997. View at Google Scholar · View at Scopus
  155. Á. Dán, T. Molnár, I. Biksi, R. Glávits, M. Shaheim, and B. Harrach, “Characterisation of Hungarian porcine circovirus 2 genomes associated with PMWS and PDNS cases,” Acta Veterinaria Hungarica, vol. 51, no. 4, pp. 551–562, 2003. View at Publisher · View at Google Scholar · View at Scopus
  156. A. M. Martins Gomes De Castro, A. Cortez, M. B. Heinemann, P. E. Brandão, and L. J. Richtzenhain, “Genetic diversity of Brazilian strains of porcine circovirus type 2 (PCV-2) revealed by analysis of the cap gene (ORF-2),” Archives of Virology, vol. 152, no. 8, pp. 1435–1445, 2007. View at Publisher · View at Google Scholar · View at Scopus
  157. N. Ramos, S. Mirazo, G. Castro, and J. Arbiza, “Detection and molecular characterization of porcine circovirus type 2 (PCV2) from piglets with exudative epidermitis in Uruguay,” Research in Veterinary Science, vol. 93, no. 2, pp. 1042–1045, 2012. View at Publisher · View at Google Scholar · View at Scopus
  158. K.-S. Choi and J.-S. Chae, “Genetic characterization of porcine circovirus type 2 in Republic of Korea,” Research in Veterinary Science, vol. 84, no. 3, pp. 497–501, 2008. View at Publisher · View at Google Scholar · View at Scopus
  159. Z.-Z. Yang, J.-B. Shuai, X.-J. Dai, and W.-H. Fang, “A survey on porcine circovirus type 2 infection and phylogenetic analysis of its ORF2 gene in Hangzhou, Zhejiang Province, China,” Journal of Zhejiang University: Science B, vol. 9, no. 2, pp. 148–153, 2008. View at Publisher · View at Google Scholar · View at Scopus
  160. W. R. Raye, An investigation into the status of porcine circovirus in Australia [Ph.D. thesis], Murdoch University, Perth, Australia, 2004.
  161. L. J. Pérez, H. Díaz de Arce, M. I. Percedo, P. Domínguez, and M. T. Frías, “First report of porcine circovirus type 2 infections in Cuba,” Research in Veterinary Science, vol. 88, no. 3, pp. 528–530, 2010. View at Publisher · View at Google Scholar · View at Scopus
  162. S. P. Pozzi, H. Yadin, J. Lavi, M. Pacciarini, and L. Alborali, “Porcine circovirus type 2 (PCV2) infection of pigs in Israel: clinical presentation, diagnosis and virus identification,” Israel Journal of Veterinary Medicine, vol. 63, no. 4, pp. 122–125, 2008. View at Google Scholar
  163. F. Schmoll, C. Lang, A. S. Steinrigl, K. Schulze, and J. Kauffold, “Prevalence of PCV2 in Austrian and German boars and semen used for artificial insemination,” Theriogenology, vol. 69, no. 7, pp. 814–821, 2008. View at Publisher · View at Google Scholar · View at Scopus
  164. D. Tilman, K. G. Cassman, P. A. Matson, R. Naylor, and S. Polasky, “Agricultural sustainability and intensive production practices,” Nature, vol. 418, no. 6898, pp. 671–677, 2002. View at Publisher · View at Google Scholar · View at Scopus
  165. A. R. Patterson and T. Opriessnig, “Epidemiology and horizontal transmission of porcine circovirus type 2 (PCV2),” Animal Health Research Reviews / Conference of Research Workers in Animal Diseases, vol. 11, no. 2, pp. 217–234, 2010. View at Publisher · View at Google Scholar · View at Scopus
  166. M. Fenaux, P. G. Halbur, M. Gill, T. E. Toth, and X.-J. Meng, “Genetic characterization of type 2 porcine circovirus (PCV-2) from pigs with postweaning multisystemic wasting syndrome in different geographic regions of North America and development of a differential PCR-restriction fragment length polymorphism assay to detect and differentiate between infections with PCV-1 and PCV-2,” Journal of Clinical Microbiology, vol. 38, no. 7, pp. 2494–2503, 2000. View at Google Scholar · View at Scopus
  167. J. M. Mokoele, L. Janse van Rensburg, S. van Lochem et al., “Overview of the perceived risk of transboundary pig diseases in South Africa,” Journal of the South African Veterinary Association, vol. 86, no. 1, article 1197, 2015. View at Publisher · View at Google Scholar · View at Scopus
  168. D.-J. An, I.-S. Roh, D.-S. Song, C.-K. Park, and B.-K. Park, “Phylogenetic characterization of porcine circovirus type 2 in PMWS and PDNS Korean pigs between 1999 and 2006,” Virus Research, vol. 129, no. 2, pp. 115–122, 2007. View at Publisher · View at Google Scholar · View at Scopus
  169. L. Jonsson, Emerging infectious diseases: using PCV2 as a model of disease transmission dynamics at the livestock-wildlife interface in Uganda [Dissertation], Fakulteten för Veterinärmedicin och Husdjursvetenskap, Institutionen för Biomedicin och Veterinär Folkhälsovetenskap, Sveriges Lantbruksuniversitet, Uppsala, Sweden, 2013, http://stud.epsilon.slu.se/5278/7/jonsson_l_130130.pdf.
  170. V. N. Ndze, D. Cadar, A. Cságola et al., “Detection of novel porcine bocaviruses in fecal samples of asymptomatic pigs in Cameroon,” Infection, Genetics and Evolution, vol. 17, pp. 277–282, 2013. View at Publisher · View at Google Scholar · View at Scopus