Figure 3: Lack of eIF2α dephosphorylation by BV. Cells were harvested at 1 or 4 hrs PI and western blots were performed with antibody directed against phosphorylated eIF2α (Cell Signaling Technologies; Danvers, MA) as described [25]. No change in the level of phosphorylated eIF2α was evident in mock infected cells and the amount of phosphorylated eIF2α decreased from 1 to 4 hrs PI in HSV1 infected cells. In contrast, phosphorylated eIF2α levels increased in BV infected cells.