In Vitro Comparison of the Internal Ribosomal Entry Site Activity from Rodent Hepacivirus and Pegivirus and Construction of Pseudoparticles

Figure 2

RHV and RPGV IRES activity in different cell types. Schematic of the monocistronic vectors used (a). Hepa1-6 (b), MEFs (c), NIH 3T3 (d), BHK-21 (e), Huh7.5 (f), HEK 293T (h), and Vero (i) cells were transfected with the indicated plasmids. Cells were harvested and analysed by flow cytometry at 48 h.p.t.; bar graphs show MFI for mCitrine (n ≥ 8, mean ± SEM of at least three independent experiments), and representative FACS plots on the right of each graph numbers represent the MFI (mean ± SEM). The dashed line represents the fluorescent output of the control plasmid containing a scrambled sequence in place of the viral 5′ UTR.

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