In Vitro Comparison of the Internal Ribosomal Entry Site Activity from Rodent Hepacivirus and Pegivirus and Construction of Pseudoparticles

Figure 4

Expression of RHV envelope proteins. Schematic of the RHV1 envelope expression vector and western blot of cell lysate at 48 h.p.t. from transfected HEK 293T (a) Intracellular immunofluorescence of 293T cells at 48 h.p.t. with RHV1 envelope expression vector, stained with flag tag in green, c-Myc tag in red, and combined with DAPI stain (b) Extracellular immunofluorescence for E2 by flag tag stain (green) and combined with WGA (red) and DAPI (blue) from 293T at 48 h.p.t. with RHV1 envelope expression vector, and images represent one slice from z-stack (c) GFP-lentivirus vector pseudotyped with RHV or RHV1 envelope proteins or VSVG were incubated on Hepa1-6 cells; GFP positive cells indicate transduction (d).

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