Hormone and Metabolite Profiles in Nesting Green and Flatback Turtles: Turtle Species with Different Life Histories
Table 1
Inter-assay, intra-assay, and sensitivity of specific enzyme linked immunoassay (ELISA) kits for thyroxine (T4), corticosterone (B), progesterone (P), testosterone (T), oestrone (E1), and dihydrotestosterone (DHT) measurements in Chelonia mydas and Natator depressus and Homo sapiens. All samples were assayed ten times.
Hormone assay
Species
Mean (SD) inter-assay variation
Mean (SD) intra-assay variation
Assay sensitivity
Reference
Thyroxine
Chelonia mydas
—
2.25 ± 0.79
0.6g/100 L
This study
Natator depressus
—
1.85 ± 1.04
Homo sapiens
7.5 ± 0.86
11.2 ± 0.7
dpc, Inc
Corticosterone
Chelonia mydas
13.17 ± 6.64
3.49 ± 1.42
0.17 ng/mL
This study
Natator depressus
16.19 ± 5.77
3.34 ± 0.25
Homo sapiens
6.45 ± 0.45
8.6 ± 0.35
Abacus Diagnostics
Progesterone
Chelonia mydas
—
5.72 ± 3.79
0.1 ng/mL
This study
Natator depressus
—
4.41 ± 1.39
Homo sapiens
10.4 ± 0.2
11.4 ± 1.2
dpc, Inc
Testosterone
Chelonia mydas
4.52 ± 2.40
1.40 ± 0.64
0.022 ng/mL
This study
Natator depressus
11.8 ± 4.08
4.53 ± 3.63
Homo sapiens
7.97 ± 0.88
7.3 ± 0.69
dpc, Inc
Oestrone
Chelonia mydas
4.52 ± 2.40
1.40 ± 0.65
0.01 pg/mL
This study
Natator depressus
11.8 ± 4.08
4.53 ± 3.65
Homo sapiens
7.67 ± 0.73
9.5 ± 1.4
dpc, Inc
Dihydrotestosterone
Chelonia mydas
—
9.22 ± 3.47
0.006 ng/mL
This study
Natator depressus
—
4.49 ± 1.38
Homo sapiens
6.92 ± 2.29
8.5 ± 1.86
dpc, Inc
Note: inter-assay variation was not determined in P, T4, and DHT ELISA kits for either turtle species.
