7th International Symposium on Applied Bioinorganic Chemistry Guanajuato, Mexico, April 2003View this Special Issue
Alejandro López-Cortés, Sergey Bursakov, Angelo Figueiredo, Anders E. Thapper, Smilja Todorovic, José J. G. Moura, Bernard Ollivier, Isabel Moura, Guy Fauque, "Purification and Preliminary Characterization of Tetraheme Cytochrome and Adenylylsulfate Reductase from the Peptidolytic Sulfate-Reducing Bacterium Desulfovibrio aminophilus DSM 12254", Bioinorganic Chemistry and Applications, vol. 3, Article ID 862482, 11 pages, 2005. https://doi.org/10.1155/BCA.2005.81
Purification and Preliminary Characterization of Tetraheme Cytochrome and Adenylylsulfate Reductase from the Peptidolytic Sulfate-Reducing Bacterium Desulfovibrio aminophilus DSM 12254
Two proteins were purified and preliminarily characterized from the soluble extract of cells (310 g, wet weight) of the aminolytic and peptidolytic sulfate-reducing bacterium Desulfovibrio (D.) aminophilus DSM 12254. The iron-sulfur flavoenzyme adenylylsulfate (adenosine 5'-phosphosulfate, APS) reductase, a key enzyme in the microbial dissimilatory sulfate reduction, has been purified in three chromatographic steps (DEAE-Biogel A, Source 15, and Superdex 200 columns). It contains two different subunits with molecular masses of 75 and 18 kDa. The fraction after the last purification step had a purity index of 5.34, which was used for further EPR spectroscopic studies. The D. aminophilus APS reductase is very similar to the homologous enzymes isolated from D. gigas and D. desulfuricans ATCC 27774. A tetraheme cytochrome (His-heme iron-His) has been purified in three chromatographic steps (DEAE- Biogel A, Source 15, and Biogel-HTP columns) and preliminarily characterized. It has a purity index of 2.9 and a molecular mass of around 15 kDa, and its spectroscopic characterization (NMR and EPR) has been carried out. This hemoprotein presents similarities with the tetraheme cytochrome from Desulfomicrobium (Des.) norvegicum (NMR spectra, and N-terminal amino acid sequence).
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