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Bioinorganic Chemistry and Applications
Volume 2006, Article ID 23560, 10 pages
http://dx.doi.org/10.1155/BCA/2006/23560

Kinetic Study of DNA Modification by Phthalocyanine Derivative of the Oligonucleotide

1Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Novosibirsk 630090, Russia
2Department of Natural Sciences, Novosibirsk State University, Novosibirsk 630090, Russia
3Institute of Human Ecology, Siberian Branch of the Russian Academy of Sciences, Kemerovo 650099, Russia

Received 14 May 2006; Revised 26 July 2006; Accepted 13 September 2006

Copyright © 2006 Alexandra A. Kuznetsova et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Design of chemically modified oligonucleotides for regulation of gene expression has attracted considerable attention over the last decades. One actively pursued approach involves antisense or antigene constructs carrying reactive groups, many of these based on transition metal complexes. The complexes of Co(II) with phthalocyanines are extremely good catalysts of oxidation of organic compounds with molecular oxygen and hydrogen peroxide. In this study, we have investigated the kinetics and thermodynamics of sequence-specific modification of DNA with deoxyribooligonucleotide linked to Co(II)-tetracarboxyphthalocyanine (PtcCo(II)) in the presence of H2O2.