Bioinorganic Chemistry and Applications

Bioinorganic Chemistry and Applications / 2007 / Article

Research Article | Open Access

Volume 2007 |Article ID 036497 | https://doi.org/10.1155/2007/36497

M. C. Prabhakara, B. Basavaraju, H. S. Bhojya Naik, "Co(III) and Ni(II) Complexes Containing Bioactive Ligands: Synthesis, DNA Binding, and Photocleavage Studies", Bioinorganic Chemistry and Applications, vol. 2007, Article ID 036497, 7 pages, 2007. https://doi.org/10.1155/2007/36497

Co(III) and Ni(II) Complexes Containing Bioactive Ligands: Synthesis, DNA Binding, and Photocleavage Studies

Academic Editor: Giovanni Natile
Received08 Aug 2006
Revised24 Nov 2006
Accepted27 Nov 2006
Published08 Feb 2007

Abstract

DNA binding and photocleavage characteristics of a series of mixed ligand complexes of the type [M(bpy)2qbdp](PF6)nxH2O (where M=Co(III) or Ni(II), bpy=2.2-bipryidine, qbdp = Quinolino[3,2-b]benzodiazepine, n=3 or 2 and x=5 or 2) have been investigated. The DNA binding property of the complexes with calf thymus DNA has been investigated by using absorption spectra, viscosity measurements, as well as thermal denaturation studies. Intrinsic binding constant (Kb) has been estimated under similar set of experimental conditions. Absorption spectral studies indicate that the Co(III) and Ni(II) complexes intercalate between the base pairs of the CT-DNA tightly with intrinsic DNA binding constant of 1.3×106 and 3.1×105 M-1 in Tris-HCl buffer containing 50 mM NaCl, respectively. The proposed DNA binding mode supports the large enhancement in the relative viscosity of DNA on binding to quinolo[3,2-b]benzodiazepine. The oxidative as well as photo-induced cleavage reactions were monitered by gel electrophoresis for both complexes. The photocleavage experiments showed that the cobalt(III) complex can cleave pUC19 DNA effectively in the absence of external additives as an effective inorganic nuclease.

References

  1. R. E. Holmlin, P. J. Dandliker, and J. K. Barton, “Charge transfer through the DNA base stack,” Angewandte Chemie (International Edition in English), vol. 36, no. 24, pp. 2714–2730, 1997. View at: Publisher Site | Google Scholar
  2. S. Arounaguiri, D. Eshwaramoorthy, A. Ashokkumar, A. Dattagupta, and B. G. Maiya, “Cobalt(III), nickel(II) and ruthenium(II) complexes of 1, 10-phenanthroline family of ligands: DNA binding and photocleavage studies,” Proceedings of the Indian Academy of Sciences: Chemical Sciences, vol. 112, no. 1, pp. 1–17, 2000. View at: Google Scholar
  3. S. Leininger, B. Olenyuk, and P. J. Stang, “Self-assembly of discrete cyclic nanostructures mediated by transition metals,” Chemical Reviews, vol. 100, no. 3, pp. 853–907, 2000. View at: Publisher Site | Google Scholar
  4. S. Feng and R. Xu, “New materials in hydrothermal synthesis,” Accounts of Chemical Research, vol. 34, no. 3, pp. 239–247, 2001. View at: Publisher Site | Google Scholar
  5. M. Yuan, Y. Li, and E. Wang et al., “Hydrothermal synthesis and crystal structure of a hybrid material based on [Co4(phen)8(H2O)2(HPO3)2]4+ and a highly reduced polyoxoanion,” Journal of the Chemical Society, Dalton Transactions, no. 14, pp. 2916–2920, 2002. View at: Publisher Site | Google Scholar
  6. Y. Lu, E. Wang, and M. Yuan et al., “Hydrothermal synthesis and crystal structure of a layered vanadium phosphate with a directly coordinated organonitrogen ligand: [(V4O7)(HPO4)2(2,2bipy)2],” Journal of the Chemical Society, Dalton Transactions, no. 15, pp. 3029–3031, 2002. View at: Publisher Site | Google Scholar
  7. E. Lamour, S. Routier, J.-L. Bernier, J.-P. Catteau, C. Bailly, and H. J. Vezin, “Oxidation of CuII to CuIII, free radical production and DNA cleavage by hydroxy-salen-copper complexes. Isomeric effects studied by ESR and electrochemistry,” Journal of American Chemical Society, vol. 121, no. 9, pp. 1862–1869, 1999. View at: Publisher Site | Google Scholar
  8. A. K.-D. Mesmaeker, J.-P. Lecomte, and J. M. Kelly, “Photoreactions of metal complexes with DNA, especially those involving a primary photo-electron transfer,” in Electron Transfer II, vol. 177 of Topics in Current Chemistry, pp. 25–76, Springer, Berlin, Germany, 1996. View at: Google Scholar
  9. B. Norden, P. Lincoln, B. Akerman, and E. Tuite, “DNA interactions with substitution-inert transition metal ion complexes,” in Metal Ions in Biological Systems: Probing of Nucleic Acids by Metal Ion Complexes of Small Molecules, A. Sigel and H. Sigel, Eds., vol. 33, pp. 177–252, Marcel Dekker, New York, NY, USA, 1996. View at: Google Scholar
  10. D. S. Sigman, A. Mazumder, and D. M. Perrin, “Chemical nucleases,” Chemical Reviews, vol. 93, no. 6, pp. 2295–2316, 1993. View at: Publisher Site | Google Scholar
  11. T. Ghosh, B. G. Maiya, and A. Samanta et al., “Mixed-ligand complexes of ruthenium(II) containing new photoactive or electroactive ligands: synthesis, spectral characterization and DNA interactions,” Journal of Biological Inorganic Chemistry, vol. 10, no. 5, pp. 496–508, 2005. View at: Publisher Site | Google Scholar
  12. N. J. Turro, J. K. Barton, and D. A. Tomalia, “Molecular recognition and chemistry in restricted reaction spaces. Photophysics and photoinduced electron transfer on the surfaces of micelles, dendrimers, and DNA,” Accounts of Chemical Research, vol. 24, no. 11, pp. 332–340, 1991. View at: Publisher Site | Google Scholar
  13. F. Q. Liu, Q. X. Wang, K. Jiao, F. F. Jian, G. Y. Liu, and R. X. Li, “Synthesis, crystal structure, and DNA-binding properties of a new copper (II) complex containing mixed-ligands of 2,2-bipyridine and p-methylbenzoate,” Inorganica Chimica Acta, vol. 359, no. 5, pp. 1524–1530, 2006. View at: Publisher Site | Google Scholar
  14. T. D. Tullis, Metal-DNA Chemistry, ACS Symposium Series no. 402, American Chemical Society, Washington, DC, USA, 1989. View at: Google Scholar
  15. J. K. Barton, “Metals and DNA: molecular left-handed complements,” Science, vol. 233, no. 4765, pp. 727–734, 1986. View at: Publisher Site | Google Scholar
  16. S. Arounaguiri and B. G. Maiya, “Dipyridophenazine complexes of cobalt(III) and nickel(II): DNA-binding and photocleavage studies,” Inorganic Chemistry, vol. 35, no. 14, pp. 4267–4270, 1996. View at: Publisher Site | Google Scholar
  17. S. Arounaguiri, A. Dattagupta, and B. G. Maiya, “Redox-activated luminescence and light-induced nuclease activity of a new mixed-ligand ruthenium(II) complex,” Proceedings of the Indian Academy of Sciences: Chemical Sciences, vol. 109, no. 2, pp. 155–158, 1997. View at: Google Scholar
  18. S. Arounaguiri and B. G. Maiya, ““Electro-photo switch” and “molecular light switch” devices based on ruthenium(II) complexes of modified dipyridophenazine ligands: modulation of the photochemical function through ligand design,” Inorganic Chemistry, vol. 38, no. 5, pp. 842–843, 1999. View at: Publisher Site | Google Scholar
  19. A. A. Vlček, “Preparation of Co(dipy)2X2+ complexes (X=C1,Br,I,NO2) by controlled oxidative processes,” Journal of Inorganic Chemistry, vol. 6, no. 7, pp. 1425–1427, 1967. View at: Publisher Site | Google Scholar
  20. C. M. Harris and E. D. McKenzie, “Nitrogenous chelate complexes of transition metals—III bis-chelate copmlexes of nickel(II) with 1.10-phenanthroline, 2,2-bipyridal and analogus ligands,” Journal of Inorganic Nuclear Chemistry, vol. 29, no. 4, pp. 1047–1068, 1967. View at: Publisher Site | Google Scholar
  21. M. E. Reichmann, S. A. Rice, C. A. Thomas, and P. Doty, “A furhter examination of the molecular weight and size of deoxy pentose nucleic acid,” Journal of American Chemical Society, vol. 76, pp. 3047–3053, 1954. View at: Publisher Site | Google Scholar
  22. A. Wolfe, G. H. Shimer Jr., and T. Meehan, “Polycyclic aromatic hydrocarbons physically intercalate into duplex regions of denatured DNA,” Biochemistry, vol. 26, no. 20, pp. 6392–6396, 1987. View at: Publisher Site | Google Scholar
  23. A. Raja, V. Rajendiran, and P. U. Maheswari et al., “Copper(II) complexes of tridentate pyridylmethylethylenediamines: role of ligand steric hindrance on DNA binding and cleavage,” Journal of Inorganic Biochemistry, vol. 99, no. 8, pp. 1717–1732, 2005. View at: Publisher Site | Google Scholar
  24. H. Zhang, C.-S. Liu, X.-H. Bu, and M. Yang, “Synthesis, crystal structure, cytotoxic activity and DNA-binding properties of the copper (II) and zinc (II) complexes with 1-[3-(2-pyridyl)pyrazol-1-ylmethyl]naphthalene,” Journal of Inorganic Biochemistry, vol. 99, no. 5, pp. 1119–1125, 2005. View at: Publisher Site | Google Scholar
  25. A. Frodl, D. Herebian, and W. S. Sheldrick, “Coligand tuning of the DNA binding properties of bioorgano-metallic (η6-arene)ruthenium(II) complexes of the type [(η6-arene)-Ru(amino acid) (dppz)]n+ (dppz = dipyrido[3,2a:2,3c]phenazine), n=13,” Journal of the Chemical Society, Dalton Transactions, no. 19, pp. 3664–3673, 2002. View at: Publisher Site | Google Scholar
  26. S. Satyanarayana, J. C. Dabrowiak, and J. B. Chaires, “Tris(phenanthroline)ruthenium(II) enantiomer interactions with DNA: mode and specificity of binding,” Biochemistry, vol. 32, no. 10, pp. 2573–2584, 1993. View at: Publisher Site | Google Scholar
  27. S. Satyanarayana, J. C. Dabrowiak, and J. B. Chaires, “Neither Δ- nor Λ-tris(phenanthroline)ruthenium(II) binds to DNA by classical intercalation,” Biochemistry, vol. 31, no. 39, pp. 9319–9324, 1992. View at: Publisher Site | Google Scholar
  28. J. D. Spikes, “Photosensitization,” in The Science of Photobiology, K. C. Smith, Ed., pp. 87–112, Plenum, New York, NY, USA, 1977. View at: Google Scholar
  29. P. C. Lee and M. A. Rodgers, “Laser flash photokinetic studies of rose bengal sensitized photodynamic interactions of nucleotides and DNA,” Photochemistry and Photobiology, vol. 45, no. 1, pp. 79–86, 1986. View at: Google Scholar
  30. A. U. Khan, “Singlet molecular oxygen. A new kind of oxygen,” Journal of Physical Chemistry, vol. 80, no. 20, pp. 2219–2228, 1976. View at: Publisher Site | Google Scholar
  31. S. K. Chatopadhyay, C. V. Kumar, and P. K. Das, “Laser flash photolytic determination of triplet yields via singlet oxygen generation,” Journal of Photochemistry, vol. 24, no. 1, pp. 1–9, 1984. View at: Publisher Site | Google Scholar

Copyright © 2007 M. C. Prabhakara et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


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