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Bioinorganic Chemistry and Applications
Volume 2007 (2007), Article ID 91078, 11 pages
http://dx.doi.org/10.1155/2007/91078
Review Article

DNA-Metallodrugs Interactions Signaled by Electrochemical Biosensors: An Overview

1Dipartimento di Scienze dell’Ambiente e della Vita, Università del Piemonte Orientale, Via Bellini 25g, Alessandria 15100, Italy
2Dipartimento di Chimica, Università di Firenze, Via della Lastruccia 3, Sesto Fiorentino Florence 50019, Italy

Received 2 April 2007; Accepted 19 June 2007

Academic Editor: Viktor Brabec

Copyright © 2007 Mauro Ravera et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The interaction of drugs with DNA is an important aspect in pharmacology. In recent years, many important technological advances have been made to develop new techniques to monitor biorecognition and biointeraction on solid devices. The interaction between DNA and drugs can cause chemical and conformational modifications and, thus, variation of the electrochemical properties of nucleobases. The propensity of a given compound to interact with DNA is measured as a function of the decrease of guanine oxidation signal on a DNA electrochemical biosensor. Covalent binding at N7 of guanine, electrostatic interactions, and intercalation are the events that this kind of biosensor can detect. In this context, the interaction between a panel of antitumoral Pt-, Ru-, and Ti-based metallodrugs with DNA immobilized on screen-printed electrodes has been studied. The DNA biosensors are used for semiquantitative evaluation of the analogous interaction occurring in the biological environment.