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Bioinorganic Chemistry and Applications
Volume 2009 (2009), Article ID 702730, 7 pages
http://dx.doi.org/10.1155/2009/702730
Research Article

Synthesis, Novel Crystal Structure, and -Amyloid Binding Property of Re(I) EHIDA Analogue

Key Laboratory of Radiopharmaceuticals, Beijing Normal University, Ministry of Education, Beijing 100875, China

Received 20 May 2009; Revised 21 July 2009; Accepted 16 September 2009

Academic Editor: Giovanni Natile

Copyright © 2009 Yang Yang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A neutral compound Re(CO)3(L) (L: 2-((2-(2,6-diethylphenylamino)-2-oxoethyl)(2-ethoxy-2-oxoethyl)amino)acetic acid, an IDA analogue) has been synthesized and evaluated for in vitro imaging probes of -amyloid (A) aggregates. Results of X-ray measurement of Re(CO)3(L) demonstrated that the coordination mode of Re(CO)3(L) was different from that of classical Re/Tc(I) (tricarbonyl)-IDA analogues; the structure of Re(CO)3(L) was confirmed by means of infrared spectrum, HPLC-UV, TOF MS, and X-ray measurements (Cambridge Crystallographic Data Centre number is 732731): monoclinic , (12) Å, (8) Å, (2) Å, (0), (5), (0), and . The binding affinity for -amyloid plaques was assessed by in vitro binding assay using preformed synthetic A aggregates. The neutral compound Re(CO)3(L) showed binding affinity to A aggregates at micromolar level by fluorescence spectroscopy, and this work will encourage for further exploration of imaging agents labeled by center as probes for -amyloid plaques in vivo.