Synthesis and Biological Evaluation of New CRH Analogues

Table 2

Physicochemical properties of h/r-CRH analogues used in the present study.

	Analogues	Yield^a	HPLC^b	TLC, 𝑅f^c
		(%)	tR (min)	A	B
1	[D-Phe^12, Aib^14]CRH	41	22.79	0.22	0.39
2	[D-Phe^12, Aib^15]CRH	38	22.85	0.21	0.38
3	[D-Phe^12,Cys(Et)^21]CRH	39	23.05	0.19	0.37
4	[D-Phe^12,Cys(Pr)^38]CRH	37	23.28	0.20	0.36
5	[D-Phe^12,Cys(Et)^21, Cys(Pr)^38]CRH	33	24.07	0.18	0.35
6	[D-Phe^12,Cys(Pr)^21, Cys(Et)^38]CRH	35	23.99	0.17	0.33
7	[D-Phe^12,Cys(Pr)^21, Cys(Pr)^38]CRH	36	24.32	0.17	0.34

^aYields were calculated on the basis of the amino acid content of the resin. All peptides were at least 98% pure.
^bFor elution conditions, see the Experimental Section.
^cSolvent systems and conditions are reported in the Experimental Section.