The Δ33-35 Mutant 𝛼-Domain Containing 𝛽-Domain-Like M_𝟑S_𝟗 Cluster Exhibits the Function of 𝛼-Domain with M_𝟒S_𝟏𝟏 Cluster in Human Growth Inhibitory Factor

Table 1

Mass spectrometry analysis and metal contents of hGIF and its mutants.

Protein	𝑀𝑤(calc.)^a	𝑀𝑤(meas.)b	Metal content
hGIF	7,071	7,070.7	7±0.1
Δ33-35 mutant	6,778	6,778.6	6±0.3
β(MT3)- β(MT3)	6,741	6,743.21	6±0.2
Single β-domain	3,408	3,407.18	3±0.2

a The theoretical molecular weight.
b The measured molecular weight.