Zinc Binding Properties of Engineered RING Finger Domain of Arkadia E3 Ubiquitin Ligase
Table 1
Samples of each construct spiked with Zn prepared and analyzed.
Sample
Volume of the unknown protein sample used (l)
Final volume of the sample (ml)
Spiked Concentration of Zn (ppm)
RING-H2 construct
A
15.0
5.0
0
B
15.0
5.0
0.2
C
15.0
5.0
0.4
D
15.0
5.0
0.6
Mutant RING construct (C955R)
A
9.0
2.0
0
B
9.0
2.0
0.1
C
9.0
2.0
0.2
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