The Zn- or Cu-Thionein Character of a Metallothionein Determines Its Metal Load When Synthesized in Physiological (Metal-Unsupplemented) Conditions
Deconvoluted ESI-TOF MS spectra of the recombinant Zn(II)-MT complexes obtained from the pGEX-MT expression system with no zinc supplementation to the culture: (a) mouse MT1; (b) yeast Crs5; (c) D. melanogaster MtnB and (d) D. melanogaster MtnA. (e) MtnB was also synthesized in zinc-enriched medium, to be used as a control.
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