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Bioinorganic Chemistry and Applications
Volume 2010 (2010), Article ID 619436, 10 pages
http://dx.doi.org/10.1155/2010/619436
Research Article

Investigation on the Interactions of NiCR and NiCR-2H with DNA

1Department of Chemistry, University of the Pacific, Stockton, CA 95211, USA
2Department of Applied Chemistry, Chaoyang University of Technology, Taichung 41349, Taiwan
3T. J. L. School of Pharmacy and Health Sciences, University of the Pacific, Stockton, CA 95211, USA

Received 7 January 2010; Revised 6 April 2010; Accepted 5 May 2010

Academic Editor: Viktor Brabec

Copyright © 2010 Priyanka Chitranshi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

We report here a biophysical and biochemical approach to determine the differences in interactions of NiCR and NiCR-2H with DNA. Our goal is to determine whether such interactions are responsible for the recently observed differences in their cytotoxicity toward MCF-7 cancer cells. Viscosity measurement and fluorescence displacement titration indicated that both NiCR and NiCR-2H bind weakly to duplex DNA in the grooves. The coordination of NiCR-2H with the N-7 of -deoxyguanosine -monophosphate (-dGMP) is stronger than that of NiCR as determined by NMR. NiCR-2H, like NiCR, can selectively oxidize guanines present in distinctive DNA structures (e.g., bulges), and notably, NiCR-2H oxidizes guanines more efficiently than NiCR. In addition, UV and NMR studies revealed that NiCR is oxidized into NiCR-2H in the presence of at low molar ratios with respect to NiCR (4).