DNA Binding and Antitumor Activity of α-Diimineplatinum(II) and Palladium(II) Dithiocarbamate Complexes

Table 2

Values of Δ𝐴_max and binding parameters in the Hill equation for interaction between Pt(II) and Pd(II) complexes and CT-DNA in 20 mmol/L Tris-HCl buffer and pH 7.0.

Compound	Temperature (𝐾)	^aΔ𝐴max	^b𝑔	^c𝐾 × 10^4 (M)^−1	^d𝑛	^eError
[Pt(bpy)(Oct-dtc)]NO3	300	0.021	6	4.01	1.87	0.005
	310	0.028		1.10	1.36	0.007
[Pd(bpy)(Oct-dtc)]NO3	300	0.035	6	5.28	5.92	0.13
	310	0.058		5.86	6.18	0.14

^aChange in the absorbance when all the binding sites on CT-DNA were occupied by metal complex.
^bThe number of binding sites per 1000 nucleotides.
^cThe apparent binding constant.
^dThe Hill coefficient (as a criterion of cooperativity).
^eMaximum error between theoretical and experimental values of ν.