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Bioinorganic Chemistry and Applications
Volume 2011, Article ID 429241, 8 pages
http://dx.doi.org/10.1155/2011/429241
Research Article

DNA Interaction Studies of a New Platinum(II) Complex Containing Different Aromatic Dinitrogen Ligands

1Department of Inorganic Chemistry, Faculty of Chemistry, Razi University, Kermanshah 74155, Iran
2School of Chemistry, Damghan University, Damghan, Iran

Received 20 July 2011; Revised 12 September 2011; Accepted 13 September 2011

Academic Editor: Zhe-Sheng Chen

Copyright © 2011 Nahid Shahabadi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A new mononuclear Pt(II) complex, [Pt(DMP)(DIP)]Cl2.H2O, in which DMP is 4,4-dimethyl-2,2-bipyridine and DIP is 4,7-diphenyl-1,10-phenantroline, has been synthesized and characterized by physicochemical and spectroscopic methods. The binding interaction of this complex with calf thymus DNA (CT-DNA) was investigated using fluorimetry, spectrophotometry, circular dichroism, viscosimetry and cyclic voltametry (CV). UV-VIS spectrum showed 4 nm bathochromic shift of the absorption band at 280 nm along with significant hypochromicity for the absorption band of the complex. The intrnisic binding constant ( 𝐾 𝑏 = 2 Γ— 1 0 4 M βˆ’ 1 ) is more in keeping with intercalators and suggests this binding mode. The viscosity measurements showed that the complex-DNA interaction can be hydrophobic and confirm intercalation. Moreover, the complex induced detectable changes in the CD spectrum of CT-DNA. The fluorescence studies revealed that the probable quenching mechanism of fluorescence of the complex by CT-DNA is static quenching. The thermodynamic parameters ( Ξ” 𝐻 > 0 and Ξ” 𝑆 > 0 ) showed that main interaction with hydrogenic forces occurred that is intercalation mode. Also, CV results confirm this mode because, with increasing the CT-DNA concentration, shift to higher potential was observed.