Role of Superoxide Anions in the Redox Changes Affecting the Physiologically Occurring Cu(I)-Glutathione Complex
Figure 1
Effect of Tempol on thiol-titratable groups of the Cu(I)-[GSH]2 complex. Tempol was added to a solution containing the Cu(I)-[GSH]2 complex and the mixture incubated at 30°C for 0–60 min. Samples from such incubation were added DTNB (24 μM) and the increase in OD412 nm registered 5 min later. Results are expressed as GSH equivalents (μM). The symbols represent: (■) GSH (30 μM); (○) Cu(I)-[GSH]2 (10 μM); (●) Cu(I)-[GSH]2 (10 μM) plus Tempol (8 μM). Insert to the figure shows results obtained from carrying out the same experiment but at 37°C, during 0–30 min.
