DNA-Binding and Topoisomerase-I-Suppressing Activities of Novel Vanadium Compound Van-7
Figure 2
(a) Effect of HCPT on Topo I-mediated supercoiled pBR322 relaxation. (b) Effect of Van-7 on Topo I-mediated supercoiled pBR322 relaxation. Both of the two reactions were carried out without ATP. (c) Effect of Van-7 on Topo II-mediated supercoiled pBR322 relaxation. The reaction was carried out in presence of ATP. RLX: relaxed DNA, a circular DNA molecule that has no superhelical turns. SC: supercoiled DNA,over- or underwinding of a DNA strand.
