Research Article

DNA-Binding and Topoisomerase-I-Suppressing Activities of Novel Vanadium Compound Van-7

Figure 4

(a) Representative image of the agarose gel electrophoresis for EMSA between pBR322 DNA and Topo I. HCPT did not modulate the formation of the complex DNA/Topo 1, whereas Van-7 significantly hampered the binding of the enzyme to DNA. (b) Van-7 displaces ethidium bromide from DNA strand. The ability of Van-7 to interact with DNA was determined by a fluorescence-based ethidium bromide displacement assay. Samples contained 1 μM ethidium bromide and 1 μg DNA pBR322. Increasing concentrations of Van-7 were added, and decreasing ethidium fluorescence at 600 nm was monitored (546 nm excitation wavelength).
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(a)
756374.fig.004b
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