Immobilization of Laccase in Alginate-Gelatin Mixed Gel and Decolorization of Synthetic Dyes

Table 1

Names, classification, maximum absorbance, and removal percentage of eight synthetic dyes using immobilized enzymes (in citrate buffer 0.1 M, pH 4.5).

Name	Classification	𝜆max	Dye removal (%)	Removal rate (nmol/min)^b
Amido black 10B	Diazo	618	86.9±1.3^a	22.5±2.3
Bromothymol blue	Triphenylmethan	430	53.8±1.2	27.2±1.4
Coomassie blue G-250	Triphenylmethan	575	71.4±0.8	53.8±1.5
Crystal violet	Triphenylmethan	595	58.1±1.5	95.3±2.8
Eosin	Heterocyclic	517	32.8±1.2	20.1±1.2
Malachite green	Triphenylmethan	620	76.3±2.1	120.7±2.5
Methyl green	Triphenylmethan	630	77.5±1.5	73.4±1.4
Methyl red	Azo	522	52.1±1.8	147.3±2.8

^aMean ± SD (𝑛=3); ^bKinetic study was performed by monitoring the loss of absorbance at 𝜆_max.