Protonation Equilibria of Biologically Active Ligands in Mixed Aqueous Organic Solvents

Table 10

Protonation constants of inosine and IMP in dioxane-water media at 25°C and  mol·dm⁻³ NaCl.

System	% dioxane
Inosine IMP^c	0%	1	1	8.55
		1	1	9.09
		1	2	15.07
Inosine IMP	25%	1	1	8.85
		1	1	9.12
		1	2	15.83
Inosine IMP	37.5%	1	1	9.04
		1	1	9.24
		1	2	16.28
Inosine IMP	50%	1	1	9.14
		1	1	9.35
		1	2	16.63
Inosine IMP	62.5%	1	1	9.36
		1	1	9.39
		1	2	16.72

and are stoichiometric coefficients corresponding to ligand and H⁺, respectively; ^bdata taken from reference [83], ^cIMP = inosine 5′-monophosphate. The % is expressed as (volume/Volume). Species 11 refers to L⁻ + H⁺ LH; species 12 refers to L + 2H⁺.