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Bioinorganic Chemistry and Applications
Volume 2014, Article ID 923834, 8 pages
Research Article

Human Lung Cancer Cell Line A-549 ATCC Is Differentially Affected by Supranutritional Organic and Inorganic Selenium

1Universidad de Guanajuato, Lascurain de Retana 5, 36000 Guanajuato, GTO, Mexico
2Departamento de Fisiología, Biofísica y Neurociencias, CINVESTAV, Avenida Instituto Politécnico Nacional 2508, San Pedro Zacatenco, Gustavo A. Madero, 07360 Ciudad de México, DF, Mexico

Received 25 June 2014; Revised 10 October 2014; Accepted 13 October 2014; Published 12 November 2014

Academic Editor: Takao Yagi

Copyright © 2014 Lérida Liss Flores Villavicencio et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Staurosporine (St) was used as control to support our results of nuclear fragmentation. The results of the cells exposed to 20 µM staurosporine for 20 h, show nuclear fragmentation (DAPI) and morphological changes characteristic of apoptosis with apoptotic bodies, and altered distribution blebs actin (panel A: Fig 1b and b’. Supplementary material). Analysis of the genomic DNA shown DNA integrity and ribosomal RNA degradation compared to control cells not exposed to staurosporine (Supplementary Material, Fig. 1B). However, there is 90% cells with nuclear fragmentation (Supplementary Material, Fig. 2a), unchanged at electrophoretic pattern of DNA that usually occurs in classical apoptosis. All this supports, the results of nuclear fragmentation and DNA integrity with SSe and SeMet are not “artifacts” (Fig. 3 and 4 manuscript).

  1. Supplementary Material