Bioinorganic Chemistry and Applications / 2015 / Article / Tab 1

Research Article

N-Terminal Region of GbIspH1, Ginkgo biloba IspH Type 1, May Be Involved in the pH-Dependent Regulation of Enzyme Activity

Table 1

Kinetic parameters of IspH proteins.

SourceReducing systemKinetics parametersReference
(min−1)bS.A.
(μmolmin−1mg−1)
(μM)
(µM−1 min−1)

E. coli NADPHa0.411.60.31150.8[11]
DT-MV0.460416.319.730.7 [21]
DT-MDQc0.4112530.431.635.6

A. aeolicus DT-MV2226.65900.4[22]

P.  falciparum DT-MV1242.1393.2 [23]
NADPH10.3

GbIspH1-fullDT-MV0.173696.717.321.3This work
GbIspH1-truncated0.183477.214.923.3

NADPH was added with flavodoxin/flavodoxin reductase or ferredoxin/ferredoxin-NADP+ reductase.
MW was estimated based on the peptide sequence. IspH from E.  coli, 37.0 kDa; A. aeolicus, 31.8 kDa; P.  falciparum, 58.8 kDa.
6,7-dihydro-2,11-dimethyldipyrido1,2-a:2,1-c pyrazinium dibromide (MDQ) is a MV analog.

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