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Bioinorganic Chemistry and Applications
Volume 2018, Article ID 4924186, 9 pages
https://doi.org/10.1155/2018/4924186
Research Article

Gloriosa superba Mediated Synthesis of Platinum and Palladium Nanoparticles for Induction of Apoptosis in Breast Cancer

1Department of Microbiology, Modern College of Arts, Science and Commerce, Ganeshkhind, Pune 411016, India
2Institute of Bioinformatics and Biotechnology, Savitribai Phule Pune University, Pune 411007, India
3Indian Institute of Science, Education and Research, Pashan, Pune 411008, India
4Department of Biomedical Sciences and Engineering, BioMediTech, Tampere University of Technology, Korkeakoulunkatu 10, 33720 Tampere, Finland
5Department of Applied Physics, Defense Institute of Advanced Technology, Girinagar, Pune 411025, India
6Department of Chemical Engineering, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India
7Department of Microbiology, School of Science, RK University, Kasturbadham, Rajkot 360020, India

Correspondence should be addressed to Sougata Ghosh; moc.liamg@bbishsohg

Received 27 February 2018; Revised 16 May 2018; Accepted 26 May 2018; Published 2 July 2018

Academic Editor: Konstantinos Tsipis

Copyright © 2018 Shalaka S. Rokade et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Green chemistry approaches for designing therapeutically significant nanomedicine have gained considerable attention in the past decade. Herein, we report for the first time on anticancer potential of phytogenic platinum nanoparticles (PtNPs) and palladium nanoparticles (PdNPs) using a medicinal plant Gloriosa superba tuber extract (GSTE). The synthesis of the nanoparticles was completed within 5 hours at 100°C which was confirmed by development of dark brown and black colour for PtNPs and PdNPs, respectively, along with enhancement of the peak intensity in the UV-visible spectra. High-resolution transmission electron microscopy (HRTEM) showed that the monodispersed spherical nanoparticles were within a size range below 10 nm. Energy dispersive spectra (EDS) confirmed the elemental composition, while dynamic light scattering (DLS) helped to evaluate the hydrodynamic size of the particles. Anticancer activity against MCF-7 (human breast adenocarcinoma) cell lines was evaluated using MTT assay, flow cytometry, and confocal microscopy. PtNPs and PdNPs showed 49.65 ± 1.99% and 36.26 ± 0.91% of anticancer activity. Induction of apoptosis was most predominant in the underlying mechanism which was rationalized by externalization of phosphatidyl serine and membrane blebbing. These findings support the efficiency of phytogenic fabrication of nanoscale platinum and palladium drugs for management and therapy against breast cancer.