(a) |
(b) |
(c) |
Figure 8: Flow cytometric analysis for MCF-7 cells treated with PtNPs and PdNPs for 48 hours confirming phosphatidyl serine externalization (Annexin V-FITC binding) and cell membrane disintegration (PI staining) The dual parametric dot plots combining Annexin V-FITC and PI fluorescence show the viable cell population (lower left quadrant, Annexin V-FITC^{−} PI^{−}), the early apoptotic cells (lower right quadrant, Annexin V-FITC^{+}PI^{−}), and the late apoptotic cells (upper right quadrant, Annexin V-FITC^{+}PI^{+}). (a) Untreated cells; (b) treatment with PtNPs; (c) treatment with PdNPs. |