Gloriosa superba Mediated Synthesis of Platinum and Palladium Nanoparticles for Induction of Apoptosis in Breast Cancer

Figure 8

Flow cytometric analysis for MCF-7 cells treated with PtNPs and PdNPs for 48 hours confirming phosphatidyl serine externalization (Annexin V-FITC binding) and cell membrane disintegration (PI staining) The dual parametric dot plots combining Annexin V-FITC and PI fluorescence show the viable cell population (lower left quadrant, Annexin V-FITC⁻ PI⁻), the early apoptotic cells (lower right quadrant, Annexin V-FITC⁺PI⁻), and the late apoptotic cells (upper right quadrant, Annexin V-FITC⁺PI⁺). (a) Untreated cells; (b) treatment with PtNPs; (c) treatment with PdNPs.

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