Effects of N-Terminal and C-Terminal Polyhistidine Tag on the Stability and Function of the Thermophilic P450 CYP119

<div>Amino acid sequences of CYP119s cloned with an N-terminal (a) and C-terminal (b) His-Tag. The roman characters show the sequence of WT CYP119, and the bold amino acids are additional residues cloned. N-terminal His-tagged CYP119 also includes a thrombin cleavage site which is underlined.</div>

Bioinorganic Chemistry and Applications

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Figure 1

Figure 1: Effects of N-Terminal and C-Terminal Polyhistidine Tag on the Stability and Function of the Thermophilic P450 CYP119 