Synthesis and Characterization of Green Zinc Oxide Nanoparticles with Antiproliferative Effects through Apoptosis Induction and MicroRNA Modulation in Breast Cancer Cells

Table 1

The sequences of forward and reverse designed primers for target genes.

Gene name	Primers (5′ ⟶ 3′)	Accession number
Caspase-3	Forward: CTGGACTGTGGCATTGAGAC	NM_001224
	Reverse: GCAAAGGGACTGGATGAACC
Caspase-8	Forward: GAAAAGCAAACCTCGGGGATAC	NM_001228
	Reverse: CCAAGTGTGTTCCATTCCTGTC
β-Actin	Forward: TGAGAGGGAAATCGTGCGTG	NM_205518
	Reverse: GTTGGCATAGAGGTCTTTACGG
GAPDH	Forward: GCCTTCCGTGTTCCTACCC	NM_002046
	Reverse: TGCCTGCTTCACCACCTTC
hsa-miR-155-5p	Forward: UUAAUGCUAAUCGUGAUAGGGGUU	MIMAT0000646
hsa-miR-203a-3p	Forward: GUGAAAUGUUUAGGACCACUAG	MIMAT0000264
hsa-miR-223-3p	Forward: UGUCAGUUUGUCAAAUACCCCA	MIMAT0000280
SNORD47	Forward: CGCCAATGATGTAATGATTCTG
Universal reverse primer	Universal reverse primer was obtained from Bonyakhteh Company (Bonyakhteh, Tehran, Iran)

Abbreviations: miR: microRNA, GAPDH: glyceraldehyde 3‐phosphate dehydrogenase, and SNORD47: small nucleolar RNA.