Figure 1: Flow cytometric analysis of RPMI-8226 stained with Hoechst 33342 and gating strategy for SP analysis. Sequential gating is shown from (a) to (d). (a) Light scatter flow cytometry profile for cells based on forward scatter (FSC-A) related to size and refractive index, and side scatter (SSC-A) related to granularity. (b) Singlet gating based on FSC-H versus FSC-A. (c) Dead cells excluded by PI binding. (d) SP profile.