Innovative Analyses Support a Role for DNA Damage and an Aberrant Cell Cycle in Myelodysplastic Syndrome Pathogenesis

Figure 4

Comparative Annexin V findings in representative control versus MDS marrow. Multiparametric flow cytometry analysis of Annexin V positivity and DRAQ V in G-M and erythroid progenitors in control (top) and MDS (bottom) marrows. Major subsets of cells were identified as described in Figure 1 for control (top left) and MDS (bottom left). G-M progenitor cells from control (top middle) and MDS (bottom middle) and erythroid progenitor cells from control (top right) and MDS (bottom right) were analyzed for DRAQ V and Annexin V. The horizontal bars were used to determine levels of Annexin V positivity in MDS above the control marrow in both G-M and erythroid progenitors.