Selective Migration of Subpopulations of Bone Marrow Cells along an SDF-1α and ATP Gradient

Figure 4

Diminished stem cell populations after spontaneous migration may not be explained by marker internalization. Intracellular multicolor flow cytometric analyses were performed on BM-TNC after isolation (“fresh”), after cultivation (“incubation control”), or on BM-TNC retrieved from lower Boyden chamber after cultivation for 24 h (“spontaneous migration”). Cell populations (a) and stem cell subpopulations (b) were evaluated as shown in Figure 1. After spontaneous migration, CD34+, CD133+, and CD117+ cells (a) as well as populations expressing multiple stem cell markers (b) formed lower percentages of BM-TNC, .

(a)

(b)