Combination of Complement-Dependent Cytotoxicity and Relative Fluorescent Quantification of HLA Length Polymorphisms Facilitates the Detection of a Loss of Heterozygosity

<table>Allele loss in HLA-B visualized by RFQ (Case C). Due to the intron 4-length- polymorphism in HLA-B an amplicon of 595 bp (B*18:01) and 587 bp (B*44:03) was gained using the amplification primers 5Bin3-389cons (= 1319-42; TCC AGY ACT TCT GAG TCA CTT TAC) and 3Bin4-56 (= 1887-1908; CCT GAC CCT GCT GAA GGG CTC C). The HLA-B*44 allele is almost absent.</table>

Bone Marrow Research

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Figure 6

Figure 6: Combination of Complement-Dependent Cytotoxicity and Relative Fluorescent Quantification of HLA Length Polymorphisms Facilitates the Detection of a Loss of Heterozygosity 

Figure 6 | Combination of Complement-Dependent Cytotoxicity and Relative Fluorescent Quantification of HLA Length Polymorphisms Facilitates the Detection of a Loss of Heterozygosity 