A Simplified Method for the Aspiration of Bone Marrow from Patients Undergoing Hip and Knee Joint Replacement for Isolating Mesenchymal Stem Cells and In Vitro Chondrogenesis

Figure 1

Bone marrow aspiration, mononuclear cells (MNCs) separation, and MSCs grown in cell culture (a–e). A specimen trap used for the collection of bone marrow (a). An orthopaedic surgeon aspirating bone marrow from the femur of a patient undergoing THA (b). The surgeon showing the specimen trap after aspiration of bone marrow (c). An OR nurse showing the full view of the specimen trap along with the suction needle and the collected bone marrow (d). A flow diagram showing the procedure of MNCs separation from the bone marrow and the establishment of MSCs in cell culture (e). A graph presenting recovery of mononuclear cells (MNCs) per mL bone marrow aspirated from proximal and distal femur from the patients undergoing THA and TKA, respectively (left); a graph presenting the number of viable MSCs (expanded up to passage 3) per mL bone marrow aspirated from proximal and distal femur from the patients undergoing THA and TKA, respectively (right) (f); a graph presenting the volume of bone marrow recovered from the proximal and distal femur from the patients undergoing THA and TKA for the purpose of MNCs separation (g).

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