Optimization of Ex Vivo Murine Bone Marrow Derived Immature Dendritic Cells: A Comparative Analysis of Flask Culture Method and Mouse CD11c Positive Selection Kit Method

Table 1

Phenotype comparison of adhered and suspended cell immature DCs population derived from flask culture method and EasySep Magnet Positive Selection kit based method.

Groups	Mean ± SD percentage of expression
	Adhered cells			Suspended cells
	Method	Method	value	Method	Method	value
Yield (cells/ml)	7.0–7.5 × 10^6	6–7.0 × 10^6	-	6.0–6.5 × 10^6	6.0–6.5 × 10^6	-
Total viable cells (%)			0.0019 (S)			0.1091 (NS)
CD11c+ (%)			0.0002 (S)			0.2757 (NS)
MHC II (%)			0.0502 (NS)			0.1106 (NS)
CD86 (%)			0.1508 (NS)			0.0002 (S)
CD40 (%)			0.0048 (S)			0.0016 (S)

I: immature DCs derived from flask culture method. II: immature DCs derived from EasySep Magnet Positive Selection kit based method. Data were represented as mean ± SD and compared together using independent -test; S: significant ; NS: nonsignificant.