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Journal of Biomedicine and Biotechnology
Volume 2, Issue 1, Pages 22-30
http://dx.doi.org/10.1155/S1110724302000402
Research article

Different Forms of Vanadate on Sugar Transport in Insulin Target and Nontarget Cells

1Lady Davis Institute for Medical Research, SMBD-Jewish General Hospital, Montreal, Quebec, Canada
2Department of Biology, Concordia University, Montreal, Quebec, Canada
3Department of Medicine, McGill University, Montreal, Quebec, Canada

Copyright © 2002 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The effects of several vanadates (ie, orthovanadate, pervanadate, and two stable peroxovanadium compounds) on basal and insulin-stimulated 2-DG transport in insulin target and nontarget cell lines are reported, herein. In nontarget cells, exposure to vanadates (5×106 to 104 mol/L) resulted in 2-DG transport stimulatory responses similar to those observed in 2-DG transport post exposure to 667 nmol/L insulin alone, or insulin in combination with vanadates. In 3T3-L1 adipocytes and L6 myotubes, exposure to a vanadate compound or 67 nmol/L insulin, stimulated 2-DG transport dramatically. Again, this effect on stimulated transport was similar to 2-DG transport post-treatment with the effective vanadates in combination with insulin. While pervanadate or stable peroxovanadates stimulated 2-DG transport at 105 to 106 mol/L, orthovanadate up to 104 mol/L was not effective in stimulating 2-DG transport in any of the cell lines tested. The data indicate that the various peroxovanadates are clearly superior insulin mimetics while a more limited insulin mimesis is observed with orthovanadate over a wide variety of cell types.