BioMed Research International

BioMed Research International / 2002 / Article

Correspondence article | Open Access

Volume 2 |Article ID 419693 | https://doi.org/10.1155/S1110724302205069

Aziza Sbiti, Fatiha El Kerch, Abdelaziz Sefiani, "Analysis of Dystrophin Gene Deletions by Multiplex PCR in Moroccan Patients", BioMed Research International, vol. 2, Article ID 419693, 3 pages, 2002. https://doi.org/10.1155/S1110724302205069

Analysis of Dystrophin Gene Deletions by Multiplex PCR in Moroccan Patients

Received26 Oct 2001
Revised05 Feb 2002
Accepted06 Feb 2002

Abstract

Duchenne and Becker muscular dystrophy (DMD and BMD) are X-linked diseases resulting from a defect in the dystrophin gene located on Xp21. DMD is the most frequent neuromuscular disease in humans (1/3500 male newborn). Deletions in the dystrophin gene represent 65% of mutations in DMD/BMD patients. We have analyzed DNA from 72 Moroccan patients with DMD/BMD using the multiplex polymerase chain reaction (PCR) to screen for exon deletions within the dystrophin gene, and to estimate the frequency of these abnormalities. We found dystrophin gene deletions in 37 cases. Therefore the frequency in Moroccan DMD/BMD patients is about 51.3%. All deletions were clustered in the two known hot-spots regions, and in 81% of cases deletions were detected in the region from exon 43 to exon 52. These findings are comparable to those reported in other studies. It is important to note that in our population, we can first search for deletions of DMD gene in the most frequently deleted exons determined by this study. This may facilitate the molecular diagnosis of DMD and BMD in our country.

Copyright © 2002 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


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