The Potential Regulation of L1 Mobility by RNA Interference

Horman, Shane R.; Svoboda, Petr; Prak, Eline T. Luning

doi:https://doi.org/10.1155/JBB/2006/32713

BioMed Research International

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LINE-1 Retrotransposition: Impact on Genome Stability and Diversity and Human Disease

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Review Article | Open Access

Volume 2006 | Article ID 032713 | https://doi.org/10.1155/JBB/2006/32713

The Potential Regulation of L1 Mobility by RNA Interference

Shane R. Horman,¹Petr Svoboda,²and Eline T. Luning Prak¹

Received06 Aug 2005

Revised12 Dec 2005

Accepted20 Dec 2005

Published20 Mar 2006

Abstract

The hypothesis that RNA interference constrains L1 mobility seems inherently reasonable: L1 mobility can be dangerous and L1 RNA, the presumed target of RNAi, serves as a critical retrotransposition intermediate. Despite its plausibility, proof for this hypothesis has been difficult to obtain. Studies attempting to link the L1 retrotransposition frequency to alterations in RNAi activity have been hampered by the long times required to measure retrotransposition frequency, the pleiotropic and toxic effects of altering RNAi over similar time periods, and the possibility that other cellular machinery may contribute to the regulation of L1s. Another problem is that the commonly used L1 reporter cassette may serve as a substrate for RNAi. Here we review the L1-RNAi hypothesis and describe a genetic assay with a modified reporter cassette that detects approximately 4 times more L1 insertions than the conventional retrotransposition assay.

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Copyright © 2006 Shane R. Horman et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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