Neutrophil Secretion Induced by an Intracellular Ca<mml:math id="E1" xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:mrow><mml:mrow><mml:mn>2</mml:mn><mml:mo>+</mml:mo></mml:mrow></mml:mrow></mml:math>
Rise  and Followed by Whole-Cell Patch-Clamp Recordings 
Occurs Without any Selective Mobilization of  Different Granule
Populations

Granfeldt, Daniel; Harbecke, Olle; Björstad, Åse; Karlsson, Anna; Dahlgren, Claes

doi:https://doi.org/10.1155/JBB/2006/97803

BioMed Research International

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Research Article | Open Access

Volume 2006 | Article ID 097803 | https://doi.org/10.1155/JBB/2006/97803

Neutrophil Secretion Induced by an Intracellular Ca2+ Rise and Followed by Whole-Cell Patch-Clamp Recordings Occurs Without any Selective Mobilization of Different Granule Populations

Daniel Granfeldt,^1,2Olle Harbecke,^1,3Åse Björstad,³Anna Karlsson,¹and Claes Dahlgren¹

Received20 Dec 2005

Revised22 Mar 2006

Accepted04 May 2006

Published04 Jun 2006

Abstract

We have investigated calcium-induced secretion in human neutrophils, using a whole-cell patch-clamp technique. Mobilization of subcellular granules to the cell membrane was followed as the change in membrane capacitance (△Cm). Both the magnitude and the kinetics of the response differed between low and high concentrations of Ca2+. A sustained secretion following a short lag phase was induced by high concentrations of Ca2+ (100 μM and higher). A stable plateau was reached after 5–7 minutes at △Cm values corresponding to values expected after all specific as well as azurophil granules have been mobilized. Capacitance values of the same magnitude could be obtained also at lower Ca2+ concentrations, but typically no stable plateau was reached within the measuring time. In contrast to previous studies, we were unable to detect any pattern of secretion corresponding to a distinct submaximal response or selective mobilization of granule subsets specified by their Ca2+-sensitivity.

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Copyright

Copyright © 2006 Daniel Granfeldt et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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